| 利用DDRT-PCR研究丝羽乌骨鸡与AA肉鸡肝脏基因表达的差异 |
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| 引用本文： | 李亮,朱庆. 利用DDRT-PCR研究丝羽乌骨鸡与AA肉鸡肝脏基因表达的差异[J]. 湖北农业科学, 2007, 46(3): 341-343 |
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| 作者姓名： | 李亮 朱庆 |
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| 作者单位： | 四川农业大学动物科技学院,四川,雅安,625014 |
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| 基金项目： | 四川农业大学校科研和教改项目 |
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| 摘 要： | 利用mRNA差异显示技术研究丝羽乌骨鸡(SK)与引进的快大型肉鸡品种爱拔益加肉鸡(AA)之间肝脏组织基因表达的差异.采用非变性聚丙烯酰胺凝胶电泳结合银染方法分离差异片段,利用单链构象多态性(SSCP)技术验证差异显示片段的重扩增产物成分的单一性,采用反向Northern斑点杂交技术对获得的差异显示cDNA片段进行了差异真实性的鉴定.共获得了丝羽乌骨鸡与AA肉鸡差异表达cDNA片段38条,其中26条为丝羽乌骨鸡肝脏表达,而AA肉鸡不表达;其余12条为AA肉鸡肝脏表达,而丝羽乌骨鸡不表达.该结果为进一步研究丝羽乌骨鸡特异基因的表达探索了一种可行的方法,并积累了一定的基础资料.
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| 关 键 词： | 丝羽乌骨鸡 爱拔益加肉鸡 肝脏 基因表达 差异显示逆转录聚合酶链式反应 |
| 文章编号： | 0439-8114（2007）03-0341-03 |
| 修稿时间： | 2006-12-21 |
Study on Differential Express Genes in Liver Organ of White Silkies and AA Broilers |
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| LI Liang,ZHU Qing. Study on Differential Express Genes in Liver Organ of White Silkies and AA Broilers[J]. Hubei Agricultural Sciences, 2007, 46(3): 341-343 |
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| Authors: | LI Liang ZHU Qing |
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| Affiliation: | Animal Sci-Tech. College of Sichuan Agricultural University, Yaan 625014, Sichuan, China |
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| Abstract: | The difference of liver genes expression in Silkies and AA broilers was studied by using DDRT-PCR. The unitarity of each re-amplified differential displayed eDNA bands cut from the silver stained non-denatured polyacrylamide gels with SSCP was analyzed. The authenticity of different expression was identified by reverse Northern dot blotting and Northern dot blotting jointly. 38 different eDNA fragments were obtained from the experiment. 26 of them expressed only in the Silkies and the other 12 fragments expressed in AA broilers. This experiment showed a kind of feasible method for further study of specific expression in Silkies. |
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| Keywords: | Silkies AA broilers liver organ gene expression DDRT-PCR |
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