基于响应曲面法优化蜂胶的乙醇提取工艺

蜂胶具有优良的保健效果,一直被作为功能食品而加以应用。为了满足实际生产中提高蜂胶产品质量和生产效率的要求,在充分模拟实际生产条件的基础上,利用响应曲面法对蜂胶提取率和总黄酮含量的影响因素(提取时间、乙醇浓度、固液比、提取次数、提取温度)进行了分析。结果表明,针对本实验中采用的蜂胶毛胶样品,回归模型预测的蜂胶提取率达到38.324 6%,总黄酮含量达到199.776 0 mg/g;最佳提取条件为提取时间24 h,乙醇浓度95%,固液比1∶10,提取次数2次,提取温度为室温。为了达到指导实际生产的目的,试验结论须得到生产车间的实际检验。

Optimization of Ethanol Extraction Technology from Propolis by Response Surface Methodology

Propolis has always been utilized as functional food for its superior health effect.In order to improve the quality and production efficiency of propolis products,on the basis of fully simulating the actual production conditions,we analyzed the influencing factors(extracting time,ethanol concentration,solid-liquid ratio,distilling frequency and distilled temperature) to extraction ratio and general flavonoids content of propolis by response surface methodology(RSM).The results showed that extraction ratio and general flavoniods content predicted by regression model for the propolis samples were 38.324 6% and 199.776 mg/g,respectively in this study.The optimal ethanolic extraction conditions of propolis were as fallowing: extracting time was 24 h,ethanol concentration was 95%,solid-liquid ratio was 10 ∶ 1,extracting frequency was twice,and extracting temperature was room temperature.In order to reach the objective of guiding the production,this experiment conclusion should be inspected by the actual production workshop.