Decrease in malate dehydrogenase activities in peripheral leucocytes of type 1 diabetic dogs |
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Authors: | Arai T Nakamura M Magori E Fukuda H Sako T |
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Affiliation: | Department of Veterinary Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan. tarai@nvau.ac.jp |
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Abstract: | The activities of the enzymes involved in the malate-aspartate shuttle were measured in peripheral leucocytes of dogs with type 1 diabetes mellitus. In the diabetic dogs, fasting plasma glucose concentrations were twofold greater than control levels despite insulin injections and the activities of malate dehydrogenase (MDH), which plays a crucial role in the malate-aspartate shuttle, were decreased remarkably. The cytosolic ratio of MDH/lactate dehydrogenase (LDH) activity (M/L ratio) in leucocytes of the diabetic dogs was significantly lower than that of normal control dogs. The decrease of the M/L ratio appeared to reflect depression of energy metabolism in leucocytes of the diabetic dogs. The M/L ratio may be a useful parameter to evaluate metabolic conditions in diabetic dogs. |
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