| 复方杠板归胶囊中芍药苷含量测定(英文) |
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| 引用本文： | 屈相玲,;曹佩雪,;马四补. 复方杠板归胶囊中芍药苷含量测定(英文)[J]. 农业科学与技术, 2014, 0(7): 1145-1146 |
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| 作者姓名： | 屈相玲, 曹佩雪, 马四补 |
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| 作者单位： | [1]贵阳中医学院第二附属医院,贵州贵阳550003; [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002; [3]贵阳中医学院,贵州贵阳550001 |
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| 基金项目： | 贵州省中医药管理局中医药、民族医药科学技术研究课题（合同编号QZYY2011-40）. |
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| 摘 要： | [目的]建立测定复方杠板归胶囊中芍药苷含量的高效液相色谱法。[方法]采用Sinochrom ODS-AP柱(5μm,4.6×250 mm),流动相为甲醇-0.3%磷酸溶液(35∶65),柱温30℃,流速0.8 ml/min,检测波长230 nm。[结果]芍药苷含量在0.0405 4-0.243 2μg/μl(r=0.999 99)与峰面积呈良好线性关系,平均加样回收率为98.89%(RSD为2.17%)。[结论]该方法快捷,方便,重复性好,可作为该复方的质量控制指标。
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| 关 键 词： | 复方杠板归胶囊 芍药苷 高效液相色谱法 |
Assay of Peoniflorin in Gangbangui Capsule |
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| Affiliation: | Xiangling QU, Peixue CAO, Sibu MA( 1. The Second Affiliated Hospital of Guiyang College of TCM, Guiyang 550003, China; 2. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China; 3. Guiyang College of Traditional Chinese Medicine, Guiyang 550001, China) |
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| Abstract: | [Objective] We aimed to establish a rapid determination method of peoniflorin in Gangbangui capsule by using HPLC.[Method] Sinochrom ODS-AP column （5 μm,4.6 mm× 250 mm） was used in our research.The mobile phase was methanol-0.3％ phosphoric acid （35：65）.The column temperature was 30 ℃.The flow rate was 0.8 ml/min.The detection wavelength was 230 nm.[Result] The concentration of peoniflorin in Gangbangui capsule ranged between 0.040 54 and 0.2432 μg/μl and a good linear relation between concentration and peak area was shown （r=0.999 99）.The average recovery was 98.89％ （RSD=2.17％）.[Conclusion] The method was fast,easy and reproducible and could be used as a quality indicator of the compound. |
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| Keywords: | Gangbangui capsule Peoniflorin HPLC |
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