| 木立芦荟叶片愈伤组织诱导过程中防褐化的研究 |
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| 引用本文： | 李桂荣,赵润洲,梁玉陈.木立芦荟叶片愈伤组织诱导过程中防褐化的研究[J].西南农业学报,2007,20(4):702-705. |
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| 作者姓名： | 李桂荣 赵润洲 梁玉陈 |
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| 作者单位： | 河南科技学院园艺系,河南,新乡,453003 |
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| 摘 要： | 以木立芦荟叶片作外植体,培养于附加6-BA 2.0 mg/L 2,4-D 0.5 mg/L的MS培养基上。添加不同浓度的活性炭及维生素C附加物成分,研究木立芦荟在叶片愈伤组织诱导过程中,各种附加物成分对防止褐化的影响。结果表明:活性炭和维生素C均可有效防止褐化,2.0 g/L的活性炭或0.2 g/L的维生素C能不同程度的减轻褐化,效果最好,活性炭和维生素C配合使用时,以2.0 g/L活性炭与0.1 g/L维生素C配合使用时最理想。
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| 关 键 词： | 木立芦荟 组织培养 褐化 活性炭 维生素C |
| 文章编号： | 1001-4829（2007）04-0702-04 |
| 修稿时间： | 2006-03-18 |
Study on browning control of leaf callus inducement in tissue culture of Aloe arborescens Mill. |
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| LI Gui-rong,ZHAO Run-zhou,LIANG Yu-cheng.Study on browning control of leaf callus inducement in tissue culture of Aloe arborescens Mill.[J].Southwest China Journal of Agricultural Sciences,2007,20(4):702-705. |
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| Authors: | LI Gui-rong ZHAO Run-zhou LIANG Yu-cheng |
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| Institution: | Department of Horticulture, Henan College of Science and Technology, Henan Xinxiang 453003, China |
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| Abstract: | Leaf of Aloe arborescens Mill.was cultured on MS 6-BA 2.0 mg/L 2,4-D 0.5 mg/L.Their effect was studied when we appended different concentration of active carbon and Vc to the medium.The result showed that the active carbon and Vc all could effectively prevent Aloe arborescens Mill.from browning.The optimal concentration of active carbon was 2.0 g/L.0.2 g/L Vc was optimal.The optimal concentration of active carbon together with Vc was 2.0 g/L,and the Vc was 0.1g/L. |
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| Keywords: | Aloe arborescens Mill tissue culture browning active carbon Vc |
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