双孢菇对壳聚糖保鲜剂的响应及其保鲜技术优化

以3种不同分子质量的壳聚糖为主要原料,以新采收的双孢菇为试验材料,研究了无毒无残留无公害绿色双孢菇保鲜方法.通过测定不同处理的双孢菇的失水率、硬度、腐烂度、开伞率和褐变度的变化,对其保鲜技术进行了优化.探讨了壳聚糖保鲜剂在双孢菇贮藏过程中所起的生理生化作用.结果表明,在贮藏过程中,经壳聚糖处理的双孢菇,其失水率、硬度、腐烂度、开伞率和褐变度均显著低于对照;子实体可溶性蛋白、总糖、还原糖的均含量明显高于对照;还原糖、MDA的含量、相对电导率均低于对照;SOD和CAT的活性明显高于对照;壳聚糖能维持细胞膜的完整性,避免了PPO与酚类物质的接触,从而抑制了双孢菇的褐变发生

Responses of Agaricus bisporus on chitosan antistaling agent and optimization of its refreshing technology

With three kinds of molecular weight chitosan as the main raw materials,with the new harvest Agaricus bisporus as the test materials,we researched the non-toxic,no residue,pollution-free and green refreshing methods.Through the determination of the changes of water loss rate,hardness,decay degrees,deployment rate and browning degree of Agaricus bisporus in various processes,we optimized its refreshing technology and discussed the physiological and biochemical role of chitosan antistaling agent in the storage process of Agaricus bisporus.The results showed that: in the storage process,the water loss rate,hardness,decay degrees,deployment rate and browning degree of Agaricus bisporus were obviously higher than those of control;the soluble protein and total sugar of the fruit body were obviously higher than those of contrast;the content of reducing sugar and MDA,and relative conductivity were lower than those of contrast;the activity of SOD and CAT were obviously higher than those of contrast;chitosan could maintain the integrity of the cell membrane to avoid the contact of PPO and phenolic compounds,thus it could restrain the appearance of browning.