构树皮化学成分鉴定及其抗菌活性研究

目的]研究桑科构树属植物构树（Broussonetia papyrifera）皮中的化学成分。方法]干燥的构树皮用95%乙醇提取,依次用石油醚、乙酸乙酯、正丁醇萃取,对乙酸乙酯部分采用硅胶、Sephadex LH-20柱色谱进行分离纯化,通过波谱数据分析（MS,^1H-NMR,^13C-NMR）进行结构鉴定。结果]从乙酸乙酯部分分离鉴定了9个化合物。其中,6个黄酮类化合物分别为：kazinol B（1）,7,4′-dihydroxy-3′-prenylflavan（2）,7,3′-dihydroxy-4′-methoxyflavan（3）,kazinol A（4）,3′-（3-methylbut-2-enyl）-3′,4′,7-trihydroxyflavane（5）和BroussochalconeA（6）;2个三萜分别为：齐墩果酸（7）和augustic acid（8）;胡萝卜苷（9）。抗菌试验显示,化合物1,2,5,7对口腔微变异链球菌（S.mATCC25175）、放线菌（A.n ATCC12104）、牙龈卟啉单胞菌（P.g ATCC33277）和厌氧具核梭菌（F.n ATCC10953）均具有较好的抑制作用。结论]化合物3、7、8为首次从构树树皮中分离得到。

Studies on Chemical Constituents of Cortex of Broussonetia papyrifera

Objective]To investigate the chemical constituents from the cortex of Broussonetia papyrifera. Method]The cortex of Broussonetia papyrifera were extracted with 95% ethanol. The EtOH extract was dispersed in H2O,and extracted with petroleum,EtOAc and BuOH successively. The EtOAc fraction was isolated and purified by column chromatography on silica gel and Sephadex. The compounds were identified on the basisi of spectral analyses(including MS,1H-NMR,1C-NMR). Result]Nine compounds were isolated from from th...