The relationship between phagocytosis and intracellular killing of Staphylococcus aureus by bovine neutrophils |
| |
| Authors: | M R Williams N Craven T R Field K J Bunch |
| |
| Affiliation: | 1. Institute of Veterinary Immunology & Engineering, National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;3. Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China;4. College of Veterinary Medicine, Nanjing Agriculture University, Nanjing, China;5. College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, Jiangsu 210023, China;1. Companion Animal Cancer Research Unit, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, Thailand;2. Department of Veterinary Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand;1. Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California Davis, 1 Shields Ave, Davis, CA, United States;2. Division of Veterinary Pathology. Department of Biomedical Sciences & Pathobiology Virginia Tech, Blacksburg, VA, 24060, United States;1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China;3. College of Life Science and Technology, Xinjiang University, Urumqi 830046, China;4. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;5. Department of Pathobiology, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan;1. M-team and Mastitis and Milk Quality Research Unit, Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium;2. GD Animal Health, PO Box 9, 7400 AA Deventer, the Netherlands;3. Department of Animal Sciences, Wageningen University, 6708 PB Wageningen, the Netherlands;4. Department of Population Health Sciences, Utrecht University, 3584 CL, Utrecht, the Netherlands;5. Flanders Research Institute for Agriculture, Fisheries, and Food (ILVO), Technology and Food Science, Agricultural Engineering, B-9820 Merelbeke, Belgium;6. Department of Pathology, Bacteriology, and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium |
| |
| Abstract: | Neutrophil (PMN) function was compared in different cows by tests of phagocytosis, total bacterial kill, intracellular survival of Staphylococcus aureus and also by luminol-dependent chemiluminescence (CL) during phagocytosis of preopsonized zymosan. Phagocytosis was estimated by direct counts of extracellular survivors after differential sedimentation of PMN and also derived from measurement of intracellular survivors after treatment with lysostaphin. This treatment eliminated bacteria which adhered to the surface of PMN or which sedimented on centrifugation. The results obtained by the two methods were found to be highly correlated. The estimates of total bacterial survival were highly correlated with both phagocytosis and intracellular survival of Staph. aureus (P < 0·01). PMN from cows with a high rate of phagocytosis generally killed internalized Staph. aureus more efficiently. When the ratio of bacteria to PMN was increased from 1:3 to 11:3, the number of phagocytosed bacteria increased proportionately but intracellular killing was impaired. Therefore increased phagocytosis per se did not stimulate intracellular killing of Staph. aureus.Intracellular survival but not phagocytosis of Staph. aureus was also correlated with reduced CL by bovine PMN. This implies that oxidative pathways are required for the efficient destruction of Staph. aureus. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
