| 蜂胶黄酮的超声波提取工艺研究 |
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| 引用本文： | 潘秋月,李英华,周晓红,杨凤杰. 蜂胶黄酮的超声波提取工艺研究[J]. 蜜蜂杂志, 2011, 31(12): 10-12 |
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| 作者姓名： | 潘秋月 李英华 周晓红 杨凤杰 |
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| 作者单位： | 浙江经贸职业技术学院,浙江杭州,310018 |
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| 基金项目： | 浙江省新苗人才项目(2010R441001); 蜂产业共性关键技术研究与集成应用(2010GA701004) |
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| 摘 要： | 通过单因素实验研究了蜂胶黄酮的超声波提取工艺，并对蜂胶中黄酮类化合物的清除活性氧自由基的能力进行测定。结果表明蜂胶黄酮的最佳提取工艺为：75％乙醇作为溶剂，料液比为1：10，提取时间为20min，超声波提取2次，温度为60cc，此时黄酮提取含量最高，达到5．046mg／g。蜂胶中黄酮提取液稀释100倍后对DPPH活性氧自由基的清除能力和Vc的清除能力相当，蜂胶黄酮是比较有开发潜力的抗氧化剂。
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| 关 键 词： | 蜂胶 黄酮 超声波提取 抗氧化 DPPH自由基 |
The Ultrasonic Extraction Methods of Total Flavonoids in Propolis |
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| PAN Qiu-yue,LI Ying-hua,ZHOU Xiao-hong,YANG Feng-jie. The Ultrasonic Extraction Methods of Total Flavonoids in Propolis[J]. Journal of Bee, 2011, 31(12): 10-12 |
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| Authors: | PAN Qiu-yue LI Ying-hua ZHOU Xiao-hong YANG Feng-jie |
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| Affiliation: | PAN Qiu-yue,LI Ying-hua,ZHOU Xiao-hong,YANG Feng-jie (College of zhejiang Economic & Polytechnic,hangzhou,zhejiang,310018) |
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| Abstract: | To study the ultrasonic conditions and moni- tor the content of flavonoids in propolis, the monothetic experimental method was used to determine the best condi- tions for extraction of flavonoids in propolis by using ul- trasonic. The anti-oxidant activity was determined by us- ing DPPH. The optimum extract of total flavonoids was ob- tained under the conditions of 60℃, ratio of propolis to solvent 1：10, ethanol concentration 75% and ultrasonic ex- tracting for 20min, continuous 2 time. With the optimized technology, the content of total flavonoids in the final product was 5.046mg/g. Extraction of propolis flavonoids dilution （multiple 100 diluted solution） has the same an- ti-oxidant activity with the same concentration VC. Propo- lis has a bright future for antioxidant exploitation. |
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