鸡EX-FABP基因PCR-SSCP分析

以AA白鸡、广西鸡、E1矮小鸡品系为试验材料,采用PCR-SSCP技术对鸡细胞外脂肪酸结合蛋白基因(EX-FABP)的2个片段进行PCR扩增,分析了EX-FABP基因在3个鸡品系的多态性。结果表明:EX-FABP基因在2对引物扩增片段中均存在PCR-SSCP多态性。对于引物1扩增片段,3个鸡品系均检测到BB基因型,AA基因型只出现在AA白鸡中;而且B等位基因频率在3个鸡品系中均明显高于A等位基因频率。对于引物2扩增片段,3个鸡品系均检测到HH和Hh基因型,hh基因型只出现在E1矮小鸡中;H等位基因频率在3个品系中均明显高于h等位基因频率。引物1和引物2的多态性片段测序分析表明,EX-FABP基因第641位点和645位点分别发生了单碱基的转换(G-A和T-C),第3264位点发生了转换(T-C)。

PCR-SSCP analysis of extracellular fatty acid binding protein gene in chicken

The polymorphism of Extracellular Fatty Acid Binding Protein (EX-FABP) gene in AA breed, Guang Xi breed and E1 Ai Xiao breed was analyzed by PCR-SSCP. The results indicated that there were three genotypes (AA, AB and BB) detected by primer 1 BB genotype was detected in all the three breeds. AA genotype was only detected in AA breed. Frequency of B allele was obviously higher than that of A allele in all the three breeds. There were also three genotypes (HH, Hh and hh) were detected by primer 2 HH and Hh genotype were detected in all the three breeds; hh genotype was only detected in E1 Ai Xiao breed. Frequency of H allele was obviously higher than that of h allele in all the three breeds. The polymorphic fragments amplified by both primers were cloned and sequenced. The sequencing results showed that there was transition(T-C) at 645 site and(G-A)at 641 site,one transition(T-C)at 3 264 site of EX-FABP gene in chicken, respectively.