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The production of homozygous diploid plants of Solanum verrucosum by tissue culture techniques
Authors:M A Weatherhead  G G Henshaw
Institution:(1) Department of Plant Biology, University of Birmingham, P.O. Box 363, B15 2TT Birmingham, England
Abstract:Summary Monohaploid plants of S. verrucosum (2n=x=12) were induced in anther culture. Axillary buds from these plants were treated with colchicine in shoot tip culture for 48 hours and then transferred to a colchicine free medium. The resulting plantlets were scored for diploidy by stomatal chloroplast counts and root tip cytology and it was found that doubling of the chromosome number had occurred.
Keywords:Solanum verrucosum  Solanum tuberosum  potatoes  haploids  colchicine  tissue culture
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