The production of homozygous diploid plants of Solanum verrucosum by tissue culture techniques |
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Authors: | M. A. Weatherhead G. G. Henshaw |
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Affiliation: | (1) Department of Plant Biology, University of Birmingham, P.O. Box 363, B15 2TT Birmingham, England |
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Abstract: | Summary Monohaploid plants of S. verrucosum (2n=x=12) were induced in anther culture. Axillary buds from these plants were treated with colchicine in shoot tip culture for 48 hours and then transferred to a colchicine free medium. The resulting plantlets were scored for diploidy by stomatal chloroplast counts and root tip cytology and it was found that doubling of the chromosome number had occurred. |
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Keywords: | Solanum verrucosum Solanum tuberosum potatoes haploids colchicine tissue culture |
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