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高效液相色谱法在蛋白质分离检测中的应用
引用本文:吴少辉,张成桂,刘光明. 高效液相色谱法在蛋白质分离检测中的应用[J]. 畜牧与饲料科学, 2011, 0(8): 63-65
作者姓名:吴少辉  张成桂  刘光明
作者单位:大理学院药学院,云南大理,671000
基金项目:云南省重点产业创新工程基金项目(2008IF012)
摘    要:生物活性肽以其独特的吸收机制、极强的活性和多样性成为国内外研究的热点,其分离与分析技术是生物技术实现产业化的重中之重。高效液相色谱法以其重复性好、分辨率高等优势成为最有效、最常用的方法。主要介绍了高效液相色谱技术在多肽类物质分离、分析上的应用。

关 键 词:多肽  分离检测  高效液相色谱

Application of High Performance Liquid Chromatography in the Separation and Detection of Protein
WU Shao-hui,ZHANG Cheng-gui,LIU Guang-ming. Application of High Performance Liquid Chromatography in the Separation and Detection of Protein[J]. Animal Husbandry and Feed Science, 2011, 0(8): 63-65
Authors:WU Shao-hui  ZHANG Cheng-gui  LIU Guang-ming
Affiliation:WU Shao-hui,ZHANG Cheng-gui,LIU Guang-ming(College of Pharmacy,Dali University,Dali 671000,China)
Abstract:The unique absorption,high activity and diversity of bioactive peptide arouse people's interest in application.It is significant to study the separation and analyses of polypeptides so as contribute to industrialization.High performance liquid chromatography(HPLC) is the effective and normal method because of its good repeatability and high resolution ratio.This paper mainly introduced the application of HPLC in the separation and analysis of polypeptides.
Keywords:polypeptides  separation and detection  high performance liquid chromatography(HPLC)  
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