| 红球姜核型分析 |
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| 引用本文： | 赵丽娟,吴勋. 红球姜核型分析[J]. 畜牧与饲料科学, 2011, 0(11) |
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| 作者姓名： | 赵丽娟 吴勋 |
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| 作者单位： | 民族药用植物资源研究与利用湖南省重点实验室湘西药用植物与民族植物学湖南省高校重点实验室怀化学院生命科学系,湖南怀化,418000 |
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| 基金项目： | 湖南省教育厅一般项目(07C503) |
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| 摘 要： | 为了对红球姜(Zingibe zerumbet)的染色体进行识别,并对该物种基因组的结构进行初步研究,利用PI和DAPI组合(CPD)染色与45SrDNA探针荧光原位杂交对中期染色体进行了分析。结果显示,红球姜具有2对45SrDNA位点,分别位于第5号和第10号染色体的短臂,对应于相应染色体上的显著CPD带区。基于rDNA位点和染色体测量数据,建立了红球姜准确而详细的分子细胞遗传学核型。
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| 关 键 词： | 红球姜 CPD染色 荧光原位杂交 核型 45S rDNA |
Karyotype Analysis of Zingiber zerumbet |
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| ZHAO Li-juan,WU Xun. Karyotype Analysis of Zingiber zerumbet[J]. Animal Husbandry and Feed Science, 2011, 0(11) |
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| Authors: | ZHAO Li-juan WU Xun |
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| Affiliation: | ZHAO Li-juan,WU Xun(Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province,Key Laboratory of Xiangxi Medicinal Plants and Ethnobotany of Hunan Higher Education,Department of Life Sciences,Huaihua University,Huaihua 418000,China) |
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| Abstract: | In order to identify the chromosomes of Zingibe zerumbet and preliminarily reveal its genome structure,CPD(combined PI and DAPI)staining and fluorescence in situ hybridization(FISH) with 45S rDNA probe were applied to analyze the mitotic metaphase chromosomes.Results showed that two pairs of 45S rDNA sites were respectively detected on the short arms of the chromosome 5 and 10,corresponding to the prominent red CPD bands.The molecular cytogenetic karyotypes of Zingibe zerumbet was constructed based on the d... |
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| Keywords: | Zingiber zerumbet CPD staining fluorescence in situ hybridization(FISH) karyotype 45S rDNA |
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