| 不同冻存液对奶牛乳腺上皮细胞冻存质量的影响 |
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| 引用本文： | 杨苗,考桂兰,侯先志,高爱武,李大彪,王秀美,杨银芬. 不同冻存液对奶牛乳腺上皮细胞冻存质量的影响[J]. 畜牧与饲料科学, 2011, 0(Z1): 96-99 |
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| 作者姓名： | 杨苗 考桂兰 侯先志 高爱武 李大彪 王秀美 杨银芬 |
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| 作者单位： | 内蒙古农业大学动物科学与医学学院;内蒙古农业大学食品科学与工程学院; |
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| 基金项目： | 国家攻关项目“奶牛营养调控技术以及与其他养殖技术系统集成”(nycytx-02-05) |
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| 摘 要： | 试验旨在探究奶牛乳腺上皮细胞(bovine mammary epithelial cells,BMECs)最佳的冻存液以改善乳腺上皮细胞的冻存质量。BMECs传至第5代后分别加入以下10种不同配方的冻存液。A组:85%DMEM+10%胎牛血清+5%DMSO;B组:80%DMEM+10%胎牛血清+10%DMSO;C组:75%DMEM+10%胎牛血清+15%DMSO;D组:70%DMEM+10%胎牛血清+20%DMSO;E组:85%DMEM+5%胎牛血清+10%DMSO;F组:75%DMEM+15%胎牛血清+10%DMSO;G组:70%DMEM+20%胎牛血清+10%DMSO;H组:80%DMEM+10%胎牛血清+10%甘油;I组:70%DMEM+10%胎牛血清+20%甘油;J组:60%DMEM+10%胎牛血清+30%甘油,冻存前统一调整细胞密度到1×106个/mL冻存。分别对复苏后的细胞进行台盼蓝染色计算存活率和PI/Hoechst33258双染计算凋亡率。结果表明,BMECs经不同冻存剂冻存复苏后,细胞活力、形态学及凋亡率表现有所不同,其中B组和G组的活力和24h贴壁率较其他组高,二者的凋亡率较低,二者之间差异无显著性(P>0.05);传代后B组细胞的生长状况最好。
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| 关 键 词： | 奶牛 乳腺上皮细胞 冻存液 |
Effect of Different Cryoprotectants on the Cryopreservation Quality of Bovine Mammary Epithelial Cells |
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| YANG Miao,KAO Gui-lan,HOU Xian-zhi,GAO Ai-wu,LI Da-biao,WANG Xiu-mei,YANG Yin-fen. Effect of Different Cryoprotectants on the Cryopreservation Quality of Bovine Mammary Epithelial Cells[J]. Animal Husbandry and Feed Science, 2011, 0(Z1): 96-99 |
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| Authors: | YANG Miao KAO Gui-lan HOU Xian-zhi GAO Ai-wu LI Da-biao WANG Xiu-mei YANG Yin-fen |
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| Affiliation: | YANG Miao1,KAO Gui-lan1,HOU Xian-zhi1,GAO Ai-wu2,LI Da-biao1,WANG Xiu-mei1,YANG Yin-fen1(1.College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China,2.College of Food Science and Engineering,China) |
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| Abstract: | This paper was aimed to search an optimal cryoprotectant for improving the cryopreservation quality of bovine mammary epithelial cells.The following 10 different ingredients of cryoprotectant were added to the fifth generation of BMECs respectively,group A:85% DMEM+10% FBS+5% DMSO;group B:80% DMEM+10% FBS+10% DMSO;group C:75% DMEM+10% FBS+15% DMSO;group D:70% DMEM+10% FBS+20% DMSO;group E:85% DMEM+5% FBS+10% DMSO;group F:75% DMEM+15% FBS+10% DMSO;group G:70% DMEM+20% FBS+10% DMSO;group H:80% DMEM+10% FBS+10... |
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| Keywords: | dairy cows mammary epithelial cells cryoprotectants |
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