Immunological properties of the SLLTEVET epitope of Influenza A virus in multiple display on filamentous M13 phage |
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| Affiliation: | 1. Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;2. Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran;1. Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil;2. Departamento de Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceió, PE, Brazil;3. Departamento de Medicina Veterinária Universidade Federal de Campina Grande, Patos, PB, Brazil;4. Superintendência em Saúde, Administração do Distrito Estadual de Fernando de Noronha, Fernando de Noronha, PE, Brazil;1. Department of Small Animal Clinical Sciences, University of Florida, Gainesville, FL, USA;2. Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, USA;1. Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 402, Taiwan;2. Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 402, Taiwan;1. Pediatric Intensive Care Unit, Emma Children’s Hospital, Amsterdam, the Netherlands;2. Wageningen University and Research, Bioveterinary Research, Lelystad, the Netherlands |
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| Abstract: | Small peptides require large carriers to stimulate the humoral immune system. The filamentous phages, such as M13, have been proposed as vectors for expressing and carrying these peptides on their capsid surface. M2e 2-9 (SLLTEVET) residues of the transmembrane protein M2 of Influenza A virus are conserved and considered as a suitable target for immunization against a wide range of Influenza A virus strains. Here, M2e (2-9) sequence of Influenza A virus was fused to the N-terminus of the major coat protein gpVIII of M13 phage and was used to immunize broiler chickens. The results showed that the SLLTEVET peptide on the surface of M13 phage was expressed, the hybrid phage was immunogenic and produced specific antibodies against M2e (2-9) in broiler chickens. |
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| Keywords: | Influenza A virus M2e (2-9) M13 phage Immunization |
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