Phenotypic and genotypic presence of the Yersinia virulence plasmid do not affect the production of enterotoxin YstA by Yersinia enterocolitica strains |
| |
| Affiliation: | 1. Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), 13071, Ciudad Real, Spain;2. Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100, Espinardo, Murcia, Spain;3. Departament de Medicina i Cirugia Animals, Universidad Autónoma de Barcelona, 08193, Barcelona, Spain;1. Department of Biotechnology, Institute of Applied Sciences & Humanities, GLA University, Mathura, Uttar Pradesh, India;2. AIMT & AIB, Amity University Rajasthan, Jaipur, India;1. Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;2. Department of Medical Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;1. Post-graduate Program in Plant Biology, Federal University of Pernambuco, Recife, PE, Brazil;2. Laboratory of Applied Mycology of Cariri, Department of Biological Sciences, Regional University of Cariri, Crato, CE, Brazil;3. Laboratory of Microbiology and Molecular Biology, Department of Biological Sciences, Regional University of Cariri, Crato, CE, Brazil;4. Department of Biological Sciences, Regional University of Cariri, Crato, CE, Brazil;5. Federal University of Santa Maria, Santa Maria, RS, Brazil;1. College of Veterinary Medicine, Jilin University, Changchun, Jilin, China;2. Key laboratory for Zoonoses Research, Ministry of Education, Changchun, Jilin, China |
| |
| Abstract: | The aim of the study was to determine whether the presence of the Yersinia virulence plasmid could affect the production of enterotoxin YstA by Y. enterocolitica strains isolated from pigs which are the main source of infection for humans. The phenotypic features characteristic for the Yersinia virulence plasmid were detected on CRMOX agar in 8 out of 12 strains producing enterotoxin YstA, in 5 out of 12 doubtful strains, and in 11 out of 12 strains not producing YstA. Autoagglutination ability was detected in all 12 Y. enterocolitica strains that were positive in the suckling mice bioassay, in 11 doubtful strains and 10 negative strains. CRMOX+ colonies were generally ystA, myfA, virF and yadA positive, while CRMOX- colonies were only ystA and myfA positive. The amplicons of yadA were not detected in 2 (8.3%) out of 24 CRMOX+ and virF positive strains. The results of this study indicate that the presence of pYV does not affect the enterotoxin-producing ability of Y. enterocolitica strains. |
| |
| Keywords: | Enterotoxin YstA Phenotypic and genotypic features |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
