Chemical composition and anti-Candida potencial of the extracts of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
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| Affiliation: | 1. Post-graduate Program in Plant Biology, Federal University of Pernambuco, Recife, PE, Brazil;2. Laboratory of Applied Mycology of Cariri, Department of Biological Sciences, Regional University of Cariri, Crato, CE, Brazil;3. Laboratory of Microbiology and Molecular Biology, Department of Biological Sciences, Regional University of Cariri, Crato, CE, Brazil;4. Department of Biological Sciences, Regional University of Cariri, Crato, CE, Brazil;5. Federal University of Santa Maria, Santa Maria, RS, Brazil;1. Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), 13071, Ciudad Real, Spain;2. Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100, Espinardo, Murcia, Spain;3. Departament de Medicina i Cirugia Animals, Universidad Autónoma de Barcelona, 08193, Barcelona, Spain;1. Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA). Paraje Arroyo Seco S/N, Tandil, Buenos Aires, Argentina;2. Centro de Investigación Veterinaria de Tandil (CIVETAN)-CONICET, Paraje Arroyo Seco S/N, Tandil, Buenos Aires, Argentina;3. Laboratorio de Virología, Departamento de Producción Animal, Instituto Nacional de Tecnología Agropecuaria (INTA). Ruta 226, Km 73.5, Balcarce, Buenos Aires, Argentina;4. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, Buenos Aires, Argentina;1. Department of Biotechnology, Institute of Applied Sciences & Humanities, GLA University, Mathura, Uttar Pradesh, India;2. AIMT & AIB, Amity University Rajasthan, Jaipur, India;1. Laboratório de Produtos Naturais, Universidade Estadual do Ceará–UECE, 60740-000 Fortaleza, CE, Brazil;2. Rede Nordeste de Biotecnologia–RENORBIO, 60740-000 Fortaleza, CE, Brazil;3. Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri–URCA, 63105-000 Crato, CE, Brazil |
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| Abstract: | Phytochemical prospecting was performed by HPLC-DAD. The Inhibitory Concentration of 50% of mortality the microorganisms (IC50) was determined and a cell viability curve was obtained. Minimum Fungicidal Concentration (MFC) was determined by subculture in Sabourad Dextrose Agar. The effect of the combination extract/fluconazole was verified by microdilution, with the extracts in subinhibitory concentrations (MFC/16). Caffeic acid was the major compound of both extracts, representing 6.08% in the aqueous extract and 7.62% in the ethanolic extract. The extracts showed a fungistatic effect (MFC ≥ 16,384 μg/mL). The IC50 results demonstrated that the combination of the extracts with fluconazole were more significant than the products tested alone, with values from 4.9 to 34.8 μg/mL for the ethanolic extract/fluconazole and 5 to 84.7 μg/mL for the aqueous extract/fluconazole. The potentiating effect of fluconazole action was observed against C. albicans and C. tropicalis. In C. krusei the aqueous extract had an antagonistic effect. |
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| Keywords: | Medicinal plants Phenolic compounds Fungistatic effect Fluconazole Synergism |
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