Genetic diversity of Ehrlichia canis in dogs from Turkey inferred by TRP36 sequence analysis and phylogeny |
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| Affiliation: | 1. Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), 13071, Ciudad Real, Spain;2. Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100, Espinardo, Murcia, Spain;3. Departament de Medicina i Cirugia Animals, Universidad Autónoma de Barcelona, 08193, Barcelona, Spain;1. Comparative Tropical Medicine and Parasitology, Ludwig-Maximilians-Universität München, Leopoldstrasse 5, 80802 Munich, Germany;2. University of South Bohemia, Faculty of Science and Biology Centre of the ASCR, Parasitology Institute, České Budějovice, Czech Republic;3. Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort 0110, South Africa;4. Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X4, Onderstepoort 0110, South Africa;5. Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, Unisa, Private Bag X06, Florida 1710, South Africa;6. The Pirbright Institute, Pirbright Laboratory, Ash Road, Pirbright, Surrey GU24 0NF, UK;7. Departamento de Medicina Veterinária Preventiva, Escola de Veterinária-UFMG, Belo Horizonte, Minas Gerais, Brazil;1. Post-graduate Program in Plant Biology, Federal University of Pernambuco, Recife, PE, Brazil;2. Laboratory of Applied Mycology of Cariri, Department of Biological Sciences, Regional University of Cariri, Crato, CE, Brazil;3. Laboratory of Microbiology and Molecular Biology, Department of Biological Sciences, Regional University of Cariri, Crato, CE, Brazil;4. Department of Biological Sciences, Regional University of Cariri, Crato, CE, Brazil;5. Federal University of Santa Maria, Santa Maria, RS, Brazil |
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| Abstract: | Canine monocytic ehrlichiosis, an important tick-borne disease caused by Ehrlichia canis, is cosmopolitan but particularly prevalent in tropical and subtropical regions. In Turkey, the genetic diversity of E. canis remains undefined. The aim of this study was to characterize E. canis in naturally infected dogs from Turkey by sequencing and phylogenetic analysis of the Tandem Repeat Protein 36 (TRP36) encoded by the trp36 gene. A total of 167 archived blood samples randomly collected from municipal shelter dogs in three distinct geographic regions were analyzed for E. canis. Only ten samples (5.98%) were found positive by PCR assays target regions of the trp36 and 16S rRNA genes. Sequence analysis of Turkish E. canis TRP36 revealed five Tanden Repeat sequences (TRs) resulting to three TR genotypes: i) the previously reported US genotype composed exclusively from TRs of “TEDSVSAPA” sequence (14 or 8 TRs), ii) the previously Brazilian genotype composed exclusively from TRs of ASVVPEAE sequence (13 TRs), and iii) a novel genotype. In addition, phylogenetic analysis based on the entire sequences of TRP36 revealed that these genotypes correspond to four distinct genogroups (US genogroups I and II, Brazilian genogroup and Costa Rica-Turkey genogroup), all containing Turkish genotypes amongst other geographically distant E. canisgenotypes. |
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| Keywords: | Tandem repeat Genogroup Dog |
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