Strength and medium-term impact of HisAK70 immunization in dogs: Vaccine safety and biomarkers of effectiveness for ex vivo Leishmania infantum infection |
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| Affiliation: | 1. INMIVET, Department of Animal Health, Faculty of Veterinary Science, Complutense University of Madrid, 28040 Madrid, Spain;2. LeishmanCeres Laboratory (GLP Compliance Certificated), Parasitology Unit, Veterinary Faculty, University of Extremadura, Avenida de la Universidad s/n, 10003, Cáceres, Spain;3. Hospital Central de la Defensa Gómez Ulla, 28047 Madrid, Spain;1. Aix-Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France;2. Department of Epidemiology and Health Economics, Aix-Marseille Univ, Marseille, France;3. APHM, IHU Méditerranée Infection, UF Immunologie, Marseille, France;1. Department of Animal Management, College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;2. Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz 3120, Nueva Ecija, Philippines;3. Department of Biological Sciences, College of Arts and Sciences, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;4. Department of Animal Science, College of Agriculture, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;1. Universidade Federal Fluminense, Departamento de Microbiologia e Parasitologia, Laboratório de Bacteriologia Veterinária, Rua Professor Hernani Melo 101, Niterói, 24210-130, Brazil;2. Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Laboratório de Epidemiologia Molecular e Resistência a Antimicrobianos, Av. Prof. Orlando Marques de Paiva 87, São Paulo, 05508-010, Brazil;3. Universidade Federal de Pelotas, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Rua Gomes Carneiro 1, Pelotas, 96010-610, Brazil;1. Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil;2. Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil;1. Pediatric Intensive Care Unit, Emma Children’s Hospital, Amsterdam, the Netherlands;2. Wageningen University and Research, Bioveterinary Research, Lelystad, the Netherlands |
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| Abstract: | HisAK70 candidates have successfully been tested in cutaneous (CL) and visceral leishmaniosis (VL) mouse models. Here, we analyse different biomarkers in dog trials after a heterologous immunization strategy with a HisAK70 candidate (plasmid DNA plus adoptive transfer of peripheral blood-derived dendritic cells (DCs) pulsed with the same pathoantigen and CpG ODN as an adjuvant) to explore the antileishmanial activity in an ex vivo canine co-culture system in the presence of Leishmania infantum parasites. In the canine model, the heterologous HisAK70 vaccine could decrease the infection index in the DC-T cell co-culture system by up to 54% after 30 days and reach almost 67% after 100 days post-immunization, respectively, compared to those obtained in the control group of dogs. The observed security and potential to fight ex vivo L. infantum infection highlight a HisAK70 heterologous immunization strategy as a promising alternative to evaluate its effectiveness against canine VL. |
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| Keywords: | Zoonotic infection Visceral leishmaniosis Dogs Vaccination HisAK70 Dendritic cells Canine leishmaniosis |
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