Occurrence and genetic diversity of hemoplasmas in beef cattle from the Brazilian Pantanal,an endemic area for bovine trypanosomiasis in South America |
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| Institution: | 1. Graduate Program in Agricultural Microbiology, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;2. Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/ Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;3. Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil;1. Department of Environment, College of Science, University of Al-Qadisiyah, Iraq;2. Market Research Center and Consumer Protection, University of Baghdad, Iraq;3. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran;4. Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, University of Kufa, Iraq;1. Instituto de Biotecnología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, De Los Reseros y Dr. Nicolás Repetto s/n, 1686, Hurlingham, Buenos Aires, Argentina;2. Estación Experimental Agropecuaria Colonia Benitez, Instituto Nacional de Tecnología Agropecuaria, Marcos Briolini s/n, 3505, Colonia Benítez, Chaco, Argentina;3. Estación Experimental Agropecuaria Mercedes, Instituto Nacional de Tecnología Agropecuaria, Juan Pujol al Este s/n, 3470, Mercedes, Corrientes, Argentina;4. Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias s/n, 76230, Juriquilla, Querétaro, Mexico;1. Laboratory of Immunoparasitology, Department of Veterinary Pathology, Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, SP, Brazil;2. Department of Genetics and Animal Breeding, Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, SP, Brazil;3. Department of Veterinary Clinical Pathology, Centro Universitário Moura Lacerda, Ribeirão Preto, SP, Brazil;1. IHAP, Pathogenesis of Mycoplasma Infections, University of Toulouse, INRA, ENVT, 23 chemin des Capelles, 31076, Toulouse Cedex, France;2. Department of Veterinary Clinical Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Inada Cho, Hokkaido, 080-8555, Japan;3. Lesvet, Veterinary practice, 3 place du Champ de Bataille, 29260, Lesneven, France;4. Veterinary practice, 30 rue des écoles, 22150, Ploeuc sur Lie, France;1. Jaguar Conservation Fund/ Instituto Onça-Pintada, Caixa Postal 193, 75830-000, Mineiros-GO, Brazil;2. Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508-000, São Paulo-SP, Brazil;3. Instituto de Biociências, Departamento de Parasitologia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior, 18618-000, Botucatu-SP, Brazil;4. Instituto de Biologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, R. Ceará, s/n, 38400-902, Uberlândia-MG, Brazil;1. Pós-graduação em Microbiologia Agropecuária, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;2. Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;3. Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz/FioCruz, Rio de Janeiro, RJ, Brazil;4. Direcção de Ciências Animais, Maputo, Moçambique |
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| Abstract: | Hemotropic mycoplasmas (hemoplasmas) are Gram-negative bacteria that parasitize the erythrocyte surface of a wide variety of mammals. The present study aimed at investigating the occurrence of hemoplasmas in beef cattle in the Brazilian Pantanal, an area endemic for bovine trypanosomiasis in South America. Additionally, the objective of this study was to characterize molecularly the genotypes of the found hemoplasmas. For this purpose, blood and serum samples of 400 beef cattle were collected from five properties in Corumbá, Nhecolândia sub-region, Mato Grosso do Sul, in Midwest Brazil. Blood samples underwent DNA extraction and standard 16S rRNA gene-based PCR assays for hemoplasmas. The sequences obtained were submitted to phylogenetic inferences, distance analysis, and genotype diversity. The Indirect Enzyme-Linked Immunoabsorbent Assay (iELISA) indicated the presence of anti-Trypanosoma vivax IgG antibodies in 89.75% of the animals sampled, confirming the endemicity of said agent in the studied region. Among the 400 bovine blood samples tested, 2.25% (9/400) were positive for hemoplasmas in cPCR. The phylogenetic analysis of the obtained sequences confirmed the presence of 'Candidatus Mycoplasma haemobos' and Mycoplasma wenyonii DNA in 0.5% (2/400) and 1.75% (7/400) animals, respectively. Five genotypes of M. wenyonii and one of 'Candidatus M. haemobos' were detected among the sequenced amplicons. The present study showed low molecular occurrence of haemoplasmas in beef cattle sampled in the Brazilian Pantanal, an area endemic for bovine trypanosomiasis. Despite of the conservation of the 16S rRNA gene, there was considerable diversity of hemoplasma genotypes infecting the sampled beef cattle. |
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| Keywords: | Hemotropic mycoplasmas Beef cattle 16S rRNA Genotypic diversity Pantanal’ |
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