Influenza A viruses in birds and humans: Prevalence,molecular characterization,zoonotic significance and risk factors' assessment in poultry farms |
| |
| Affiliation: | 1. Depatment of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;2. Department of Virology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Egypt;3. The United Graduate School of Veterinary Science, Yamaguchi University, 16771-1 Yoshida, Yamaguchi-shi, 753-8515, Japan;1. Aix-Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France;2. Department of Epidemiology and Health Economics, Aix-Marseille Univ, Marseille, France;3. APHM, IHU Méditerranée Infection, UF Immunologie, Marseille, France;1. Advanced Technology Center for Aging Research, Scientific Technological Area, IRCCS INRCA, Italy;2. Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, Ancona, Italy;3. BHD Holding B.V., Amsterdam, the Netherlands;4. KBLH Holding B.V., Amsterdam, the Netherlands;1. Universidade Federal Fluminense, Departamento de Microbiologia e Parasitologia, Laboratório de Bacteriologia Veterinária, Rua Professor Hernani Melo 101, Niterói, 24210-130, Brazil;2. Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Laboratório de Epidemiologia Molecular e Resistência a Antimicrobianos, Av. Prof. Orlando Marques de Paiva 87, São Paulo, 05508-010, Brazil;3. Universidade Federal de Pelotas, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Rua Gomes Carneiro 1, Pelotas, 96010-610, Brazil;1. Department of Animal Management, College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;2. Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz 3120, Nueva Ecija, Philippines;3. Department of Biological Sciences, College of Arts and Sciences, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;4. Department of Animal Science, College of Agriculture, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;1. University of Sydney, Sydney, Australia;2. Massey University, Palmerston North, New Zealand;3. Australian Department of Agriculture, GPO Box 858, Canberra, Australian Capital Territory, Australia;4. Disease Investigation Centre Denpasar, Ministry of Agriculture, Indonesia |
| |
| Abstract: | This study aimed to investigate the prevalence of influenza A viruses in birds and humans residing in the same localities of Sharkia Province, Egypt and the risk factors' assessment in poultry farms. A total of 100 birds comprised of 50 chickens, 25 ducks and 25 wild egrets were sampled. Swab samples were collected from 65 people (50 poultry farm workers and 15 hospitalized patients). All samples were screened for the presence of influenza A viruses using isolation and molecular assays. Avian influenza viruses were only detected in chicken samples (18%) and molecularly confirmed as subtype H5. The infection rate was higher in broilers (40%) than layers (8.6%). Influenza A (H1) pdm09 virus was detected in a single human case (1.54%). All the isolated AI H5 viruses were clustered into clade (2.2.1.2) and shared a high similarity rate at nucleotides and amino acid levels. In addition, they had a multi-basic amino acid motif (ـــPQGEKRRKKR/GLFـــ) at the H5 gene cleavage site that exhibited point mutations. Chicken breed, movement of workers from one flock to another, lack of utensils' disinfection and the introduction of new birds to the farm were significant risk factors associated with highly pathogenic AI H5 virus infection in poultry farms (p ≤ 0.05). Other factors showed no significant association. The HPAI H5 viruses are still endemic in Egypt with continuous mutation. Co-circulation of these viruses in birds and pdm09 viruses in humans raises alarm for the emergence of reassortant viruses that are capable of potentiating pandemics. |
| |
| Keywords: | Avian influenza virus Birds Humans rRT-PCR pdm09 |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
