Orf virus circulation in cattle in Turkey |
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| Institution: | 1. Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, 602 Tower Rd., Ithaca, NY, 14853, United States;2. Department of Microbiology, Immunology, and Biopharmaceuticals, College of Life Sciences, National Chiayi University, No. 300, University Rd., Chiayi, 60004, Taiwan, ROC;3. Department of Animal Sciences, College of Agriculture, National Chiayi University, No. 300, University Rd., Chiayi, 60004, Taiwan, ROC;1. Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, 14155–6453 Tehran, Iran;2. Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, 5166616471 Tabriz, Iran;1. Department of Veterinary Tropical Diseases, University of Pretoria, Onderstepoort, Pretoria, South Africa;2. Department of Veterinary and Tsetse Control Services, Lusaka, Zambia;3. Department of Paraclinical Sciences, University of Pretoria, Onderstepoort, Pretoria, South Africa;4. The Pirbright Institute, Pirbright, Surrey, UK;5. National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada;6. Department of Immunology, University of Manitoba, Winnipeg, Canada;1. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 733046, PR China;2. Laboratory of Veterinary Microbiology, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, PR China;3. Department of Poultry Science, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University, Rawalpindi, 46300, Pakistan |
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| Abstract: | Orf virus (ORFV) causes contagious skin disease that mainly affects sheep and goats with zoonotic potential. However, there is not enough information about the association between ORFV and occurrence of skin disease in cattle. The present study describes outbreaks of ORFV infection in cattle in different provinces that are located in the Aegean, Central Anatolian and Mediterranean regions of Turkey. During the months of June and August 2017, vesicular fluid and scab samples were collected from cattle which had proliferative skin lesions. First, presence of lumpy skin disease virus (LSDV) and bovine herpesvirus 2 (BoHV-2, known as the causative agent of pseudo-lumpy skin disease) were investigated by real time PCR and PCR, respectively. Then, samples tested for the presence of parapoxviruses by PCR using primers specific to major envelope protein gene (B2L). Parapoxvirus DNA was detected in investigated samples whereas LSDV and BoHV-2 DNA were not detected. The analysis of the B2L gene sequences revealed that cattle were infected with ORFV. The isolates in the present study shared 100% sequence identity at the nucleotide and amino acid level when compared with previously characterised Turkish field ORFV isolates from goats in 2016. Results of the study show unusual infection of cattle with ORFV, and suggest that ORFV jumps the host species barrier from goats to cattle. |
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| Keywords: | Parapoxvirus Orf virus Cattle Skin disease B2L gene Turkey |
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