Molecular genotyping and serological evaluation of Toxoplasma gondii in mothers and their spontaneous aborted fetuses in Southwest of Iran |
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| Institution: | 1. The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;2. Department of Veterinary Parasitological Diseases, Faculty of Applied Biological Science, 1–1 Yanagido, Gifu 501-1193, Japan;3. National Research Center for Protozoan Diseases (NRCPD), Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan;4. Livestock and Poultry Research Centre, College of Basic and Applied Sciences, University of Ghana, P.O. Box LG 25, Legon, Accra, Ghana;5. Department of Animal Science, College of Basic and Applied Sciences, University of Ghana, P.O. Box LG 25, Legon, Accra, Ghana;6. Department of Animal Biology and Conservation Science, University of Ghana, P.O. Box LG 25, Legon, Accra, Ghana;7. Department of Microbiology, Faculty of Applied Biological Science, 1–1 Yanagido, Gifu 501-1193, Japan;8. Wildlife Research Center of Kyoto University, 2-24 Tanaka-Sekiden-cho, Kyoto 606-8203, Japan;9. Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan;10. Mocky Poultry Practice, Shinmeidai 2-5-33-810, Hamura, Tokyo 205-0023, Japan;11. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan;12. Center for Highly Advanced Integration of Nano and Life Sciences, Gifu University (G-CHAIN), 1-1 Yanagido, Gifu 501-1193, Japan |
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| Abstract: | BackgroundGiven the lack of routine screening and the high prevalence of toxoplasmosis in pregnant women in Iran, the current study aimed to find out the rate and features of Toxoplasma gondii infection in the spontaneously aborted human fetuses in Kohgiluyeh and Boyer-Ahmad Province, Southwestern Iran.MethodsThis cross-sectional study was performed on 100 spontaneously aborted fetuses’ tissues and their mother blood samples. The mothers’ sera were evaluated for anti-Toxoplasma antibodies while their buffy coat and aborted fetuses tissues were evaluated for Toxoplasma DNA. PCR product at GRA6 locus was sequenced and phylogenetic analysis was done. Likewise, quantitative Real-Time PCR was performed to find out the parasite burdens in mothers buffy coat and fetuses tissues.ResultsUsing serological method, anti-Toxoplasma IgG and IgM antibodies were detected in 7 (7%) and 3 (3%) out of 100 sera from women with spontaneous abortion. Real-time PCR method detected T. gondii DNA in the buffy coat of one seronegative and 2 (out of 3) IgM seropositive cases. None of the samples from aborted fetuses were infected with T. gondii. BLAST and phylogenetic analysis showed that the sequenced isolates belonged to type I of T. gondii and two identified T. gondii isolates were taxonomically grouped into one clade.ConclusionOur findings revealed type I genotype of T. gondii in two mothers with spontaneous abortion, without fetus involvement. It is necessary to examine more aborted fetuses’ samples from different geographical areas to determine the association between Toxoplasma genotype and abortion. |
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| Keywords: | Pregnancy Aborted fetus genotype Iran |
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