Salmonella enterica serotype typhimurium and S. Stanley differ in genomic evolutionary patterns and early immune responses in human THP-1 cell line and CD14+ monocytes |
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| Affiliation: | 1. Department of Microbiology, Immunology and Biopharmaceuticals, National Chiayi University, Chiayi City, No. 300, University Rd, Chiayi, 60004, Taiwan, ROC;2. Graduate Institute of Medical Sciences, National Defense Medical Center, 114 No. 161, Sec. 6, Minquan E. Rd., Neihu Dist., Taipei City, 114, Taiwan, ROC;3. The Central Region Laboratory, Center of Research, Diagnostics, and Vaccine Development, Centers for Disease Control, No. 30, Wenxin S. 3rd Rd., Nantun Dist., Taichung, 40856, Taiwan, ROC;1. Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), 13071, Ciudad Real, Spain;2. Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence ‘Campus Mare Nostrum’, University of Murcia, 30100, Espinardo, Murcia, Spain;3. Departament de Medicina i Cirugia Animals, Universidad Autónoma de Barcelona, 08193, Barcelona, Spain;1. Department of Biotechnology, Institute of Applied Sciences & Humanities, GLA University, Mathura, Uttar Pradesh, India;2. AIMT & AIB, Amity University Rajasthan, Jaipur, India;1. Advanced Technology Center for Aging Research, Scientific Technological Area, IRCCS INRCA, Italy;2. Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, Ancona, Italy;3. BHD Holding B.V., Amsterdam, the Netherlands;4. KBLH Holding B.V., Amsterdam, the Netherlands;1. Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA). Paraje Arroyo Seco S/N, Tandil, Buenos Aires, Argentina;2. Centro de Investigación Veterinaria de Tandil (CIVETAN)-CONICET, Paraje Arroyo Seco S/N, Tandil, Buenos Aires, Argentina;3. Laboratorio de Virología, Departamento de Producción Animal, Instituto Nacional de Tecnología Agropecuaria (INTA). Ruta 226, Km 73.5, Balcarce, Buenos Aires, Argentina;4. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, Buenos Aires, Argentina |
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| Abstract: | Salmonella Typhimurium and S. Stanley are the most prevalent serogroup B serovars to infect humans in Taiwan. The aim was to determine possible factors to influence the prevalence between S. Typhimurium and S. Stanley. Genotypes were determined by pulsed field gel electrophoresis (PFGE) analysis and the intracellular survival, phagocytosis, reactive oxygen species (ROS) production of human monocyte THP-1 cell and tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6), and IL-1βexpression in peripheral blood CD14+ cells after infection were analyzed. 182 S. Stanley was clonal disseminated with main pulsotypes 2 from 2004 to 2007. Overall S. Typhimurium evolved more genotypes, while S. Stanley conserved in genotypes. Human blood CD14+ monocytes expressed TNF-α, IL-6 and IL-1β differently among serovars and bacterial conditions (live vs. killed). Live S. Stanley and S. Typhimurium suppressed the TNF-α and IL-6 expression compared to killed bacteria. However, live S. Typhimurium stimulated more IL-1β expression than the killed bacteria, but S. Stanley expressed similar IL-1β levels in both conditions. Furthermore, S. Stanley and S. Typhimurium differed in intracellular survival in the THP-1 cells, an early decrease for S. Stanley, not for S. Typhimurium. Additionally, higher reactive oxygen species (ROS) production in THP-1 cells was found agsinst S. Stanley infection, not found in S. Typhimurium. However, some isolates of S. Stanley could recover from early loss to become more in the monocytes than S. Typhimurium. Difference in phagocytized number, intracellular survival, ROS production and IL-1β expression may contribute to prevalence different between two serovars. |
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| Keywords: | Cytokines Monocytes Prevalence ROS production |
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