A common conserved peptide harboring predicted T and B cell epitopes in domain III of envelope protein of Japanese Encephalitis Virus and West Nile Virus for potential use in epitope based vaccines |
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| Institution: | 1. Aix-Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France;2. Department of Epidemiology and Health Economics, Aix-Marseille Univ, Marseille, France;3. APHM, IHU Méditerranée Infection, UF Immunologie, Marseille, France;1. Department of Animal Management, College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;2. Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz 3120, Nueva Ecija, Philippines;3. Department of Biological Sciences, College of Arts and Sciences, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;4. Department of Animal Science, College of Agriculture, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines;1. Universidade Federal Fluminense, Departamento de Microbiologia e Parasitologia, Laboratório de Bacteriologia Veterinária, Rua Professor Hernani Melo 101, Niterói, 24210-130, Brazil;2. Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Laboratório de Epidemiologia Molecular e Resistência a Antimicrobianos, Av. Prof. Orlando Marques de Paiva 87, São Paulo, 05508-010, Brazil;3. Universidade Federal de Pelotas, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Rua Gomes Carneiro 1, Pelotas, 96010-610, Brazil;1. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, 350003, China;2. Fujian Animal Diseases Control Technology Development Center, Fuzhou, 350013, China;3. Animal Veterinary and Aquatic Product Bureau, Nanping, 353000, China;1. Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;2. Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran |
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| Abstract: | Japanese encephalitis virus (JEV) and West Nile virus (WNV) are two major mosquito borne flaviviruses belonging to same serocomplex. JEV is transmitted by Culex mosquitoes and the reservoir host for the virus is pigs and/or water birds. WNV is also transmitted by Culex mosquitoes and reservoir host in this case is birds. It can also be transmitted through contact with other infected animals, their blood, or other tissues. The envelope protein of these viruses is the major source of epitopes and provides protective immunity. Bioinformatics tools were used to identify conserved epitopes in the envelope protein of these viruses. A conserved peptide "TPVGRLVTVNPFV" present in both the viruses containing predicted T and B cell epitopes was found. The model of one of the predicted epitope was generated and upon docking it bound in the groove of HLA-A0201 Class I MHC molecule. Further, it was amenable to proteasomal cleavage enhancing its chances of processing by cytosolic pathway. The peptide was found to be non toxic, non allergenic and stable in mammalian cells based on database search. The population coverage was pan world and nearly 70% identity of the peptide was found in the Zika virus envelope protein. The peptide was located in the domain III of envelope protein which is the exposed domain therefore B cell receptors may recognize this peptide easily. The conserved peptide containing T and B cell epitopes can have future application for designing epitope based vaccines for both JEV and WNV. |
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| Keywords: | Flavivirus Envelope protein Epitope prediction Class I MHC Class II MHC |
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