抗除草剂基因基因枪法转化粳稻的研究 |
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引用本文: | 王景余,金成海,方秀琴,林秀峰,林秀云. 抗除草剂基因基因枪法转化粳稻的研究[J]. 吉林农业科学, 2005, 30(2): 3-6 |
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作者姓名: | 王景余 金成海 方秀琴 林秀峰 林秀云 |
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作者单位: | 北京师范大学生命科学学院植物发育生理研究室,北京,100875;吉林省农业科学院水稻研究所,吉林,公主岭,136100;吉林省农业科学院生物技术中心 |
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摘 要: | 优化了粳稻的组织培养体系;通过Gus基因的瞬间表达研究,明确了以DNA金弹到靶细胞距离为9cm,轰击2次时,PDS1000/He基因枪法转化水稻胚性愈伤组织的效率最高。应用基因枪法将抗除草剂基因(bar)转入吉林省主栽的水稻品种长白8号中,获得了可育的转基因水稻植株。经过Gus基因的组织化学分析,PCR和Southern杂交检测,证明抗除草剂基因已整合到水稻基因组中得以表达,使水稻产生对除草剂的抗性。转基因水稻中发现一例基因沉默现象。
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关 键 词: | 水稻 转基因 抗除草剂 基因枪 |
文章编号: | 1003-8701(2005)02-0003-04 |
修稿时间: | 2004-11-03 |
Transformation of Herbicide Resistant Gene to Rice via Biolistic Method |
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Abstract: | A herbicide gene(bar) was delivered into a commercial rice variety "Changbai 8" cultivated in Jilin province via biolistic method. Five fertile transgenic rice plants were obtained. Parameters of Gene Gun(PDS-1000/He) transformation of callus derived from mature embryo of Japonica rice variety were optimized by Gus gene transcient expression. When the distance between DNA-gold pellet and the target cells was 9 cm, bombard for 2 times, the transformation efficiency was the highest. It was demonstrated that bar gene had been inserted into rice genome by PCR and Southern hybridization. Three transgenic rice plants (D0-1, D0-2 and D0-3) showed herbicide resistance. Gene silence was observed in one transgenic rice plant (D0-5). |
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Keywords: | Rice Gene transformation Herbicide resistant Biolistic |
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