葡萄籽原花青素对肥胖大鼠肠粘膜的影响

为探究葡萄籽原花青素(GSP)对营养肥胖模型大鼠肠粘膜通透性的影响,本试验设置了正常、模型、低剂量、中剂量及高剂量5个处理组,其中正常组投喂普通饲料,灌胃3 mL蒸馏水;模型组和低、中、高剂量组选用32只高脂饲料诱导的肥胖大鼠,模型组灌胃蒸馏水,低、中、高剂量组分别灌胃75、162.5、250 mg·kg^-1BW·d^-1GSP,灌胃处理6周再禁食12 h后处死,经解剖后测定各处理组大鼠的生理生化相关指标。结果表明,高脂饲料造模成功率48.75%;与模型组相比,低、中、高剂量组大鼠D-乳酸、乳果糖/甘露醇排出率显著降低,回肠绒毛高度/隐窝深度、紧密连接蛋白Occludin和 ZO-1 表达量显著升高;高剂量组大鼠的回肠杯状细胞数显著升高。综上,葡萄籽原花青素可以改善肥胖应激导致的大鼠肠道粘膜屏障损伤,这在一定程度上揭示了葡萄籽原花青素减肥降脂的作用机理。

Effect of Grape Seed Procyanidins on the Intestinal Mucosal of Diet-Induced-Obesity Rats

To investigate the effects of grape seed procyanidins(GSP)on the intestinal mucosal permeability in nutritional obesity rat models. Normal diet fed rats were set as standard groups, which were given 3mL distilled water 32 high-fat diet-induced obesity rats were divided into 4 groups: control group(distilled water), low dose group,GSP,75 mg·kg^-1·BW·d^-1], middle dose group, GSP,162.5 mg·kg^-1·BW·d^-1],high dose group,GSP,250 mg·kg^-1·BW·d^-1]. All groups intragastric administration for six weeks,and stop feeding about 12 hours. Then the rats were executed, and the relevant indexes of each group were determined. The results showed that the successful rate of establishing the high-fat diet rat models was 48.75%. Compared with model group, the excretion rates of D-lactic acid and lactulose/mannitol in the GSP dose group were significantly lower; the ileal villi height/crypt depth, tight junction protein Occludin and ZO-1 expression were significantly increased, and the number of ileal goblet cells in the high-dose group was significantly higher than that in the control group. This study shows that GSP can improve the intestinal mucosal barrier injury induced by obesity stress in rats, which explained the anti-obesity effect and mechanism of GSP on the obese rats induced by high fat diet.