墨鱼缠卵腺糖蛋白的抗氧化及抑菌活性研究

为了探究墨鱼缠卵腺糖蛋白(CGG)的抗氧化活性及抑菌活性,本试验通过测定CGG的超氧阴离子自由基清除率、DPPH自由基清除率、羟基自由基清除能力、小鼠体内过氧化氢酶(CAT)活性及谷胱甘肽过氧化物酶(GSH-PX)活性等研究CGG体内外抗氧化活性,并通过抑菌试验探讨了CGG的抑菌活性。结果表明,随着CGG浓度的增大,超氧阴离子自由基清除率从17.51%增加至55.84%,DPPH自由基清除率从5.7%增加至19.5%,羟基自由基清除能力从10.38%增加至57.94%;与对照组相比, 低、中、高剂量组小鼠体内的CAT 活性分别提高了21.91%、126.29%、135.63%,GSH-PX活性分别提高了2.04%、24.69%、28.13%。抑菌试验结果表明,当CGG浓度为14 mg·mL^-1时,大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)、假单胞菌(Pseudomonadaceae)的抑菌圈分别为15.7、13.6、12.5 mm,MIC分别为1.25、7、7 mg·mL^-1,MBC分别为14、28、14 mg·mL^-1。综上表明,CGG具有一定的抗氧化和抑菌活性,但抑菌效果较弱。本研究结果为墨鱼缠卵腺的合理利用提供了一定的理论基础,同时对提高水产资源利用率有一定的参考价值。

Antioxidant and Antimicrobial Activity of Cuttlefish Gland Glycoprotein

In order to explore the antioxidant activity and antibacterial activity of cuttlefish gland glycoprotein (CGG), the superoxide anion radical scavenging rate, DPPH free radical scavenging rate, hydroxyl radical scavenging ability were measured to study the anti-oxidation actirities of CGG in ritro and in vivo. Meanwhile, the antibacterial activity of CGG was studied by antibacterial experiments. It was found that the superoxide anion radical scavenging rate, the DPPH free radical scavenging rate and the hydroxyl radical scavenging ability increased were correspondingly increased from 17.51%, 5.7% and 10.38% to 55.84%, 19.5%, 57.94% with the increased of CGG concentration, respectively. Compared with the control groups the catalase (CAT) activity in mice with low, medium and high dose were increased by 21.91%, 126.29%, 135.63%, respectively. Furthermore, the glutathione peroxidase (GSH-PX) activity in the low, medium and high dose groups was increased by 2.04%, 24.69%, and 28.13%, respectively. The antibacterial experiments showed that when the CGG concentration was 14 mg·mL^-1, the inhibition zones of Escherichia coli, Staphylococcus aureus, and Pseudomonadaceae were 15.7,13.6 and 12.5 mm, MIC were 1.25, 7, and 7 mg·mL^-1, and the MBC were 14, 28, and 14 mg·mL^-1, respectively. The results indicated that the cuttlefish gland glycoprotein squid had certain but relatively weak antioxidant and antibacterial activities. The results can provide certain theoretical basis for the rational utilization of the squid entangled gland, and reference for improving the utilization rate of aquatic resources.