壳寡糖锌配合物对氧化衰老模型小鼠的抗氧化作用

为探讨壳寡糖-锌配合物(COS-Zn²⁺)对氧化衰老模型小鼠的抗氧化作用,在体外条件下测定COS-Zn²⁺对O2·-、DPPH自由基的清除能力和Fe³⁺的还原能力,并通过D-半乳糖注射法制备氧化衰老小鼠模型,进而评价COS、ZnSO₄、COS+ZnSO₄及COS-Zn²⁺配合物对模型小鼠血清及各组织中丙二醛(MDA)含量、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性的影响。结果表明,COS-Zn²⁺对O2·-清除作用显著优于COS、ZnSO₄及二者混合物组(P<0.05);当COS-Zn²⁺浓度为10 mg·mL^-1时,其对DPPH自由基清除能力显著优于其他试验组(P<0.05);COS-Zn²⁺分子中由于-NH₂、-OH等活性基团结合了Zn²⁺,故表现出相对较弱的Fe³⁺还原能力。动物模型试验结果显示,与正常组相比,模型组小鼠体重及脏器指数均发生了显著性变化(P<0.05),COS-Zn²⁺给药对小鼠体重增加和脏器指数恢复具有一定的帮助作用,且有效降低了模型小鼠体内MDA含量,显著提高了其机体血清、肾脏和肝脏中的SOD、GSH-Px、CAT活性和T-AOC能力,尤其对血清中相关保护酶活性的改善作用显著优于COS、ZnSO₄及COS+ZnSO₄组(P<0.05)。COS-Zn²⁺表现出较好的体外抗氧化活性,同时能显著增强小鼠对氧化损伤的拮抗能力。综上,COS-Zn²⁺配合物具有较好的抗衰老及增强机体免疫功能作用。本研究结果为海洋壳寡糖及其金属修饰物的活性开发及功能应用提供了一定的理论基础与技术参考。

Antioxidant Effect of Chitooligosaccharide-zinc Complex on theOxidative Aging Mice Model

To investigate the anti-oxidative effect of chitooligosaccharides-zinc complex (COS-Zn²⁺) on the oxidative aging mice model. The ability of COS-Zn²⁺to scavenge O2·- and DPPH free radicals and the ability to reduce Fe³⁺ were measured in vitro. The oxidative aging mice model was established by D-galactose injection, and the effects of COS, ZnSO₄, COS+ZnSO₄ and COS-Zn²⁺ on malondialdehyde (MDA) content, superoxide dismutase (SOD), total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px) and catalase (CAT) activity were meaured. The scavenging effect of COS-Zn²⁺ on O2·- free radical was significantly better than that of COS, ZnSO₄ and the mixture of both (P<0.05). When the concentration of COS-Zn²⁺was 10 mg·mL^-1, DPPH radical scavenging ability was significantly better In other groups (P<0.05); When the concentration of COS-Zn²⁺was 10 mg·mL^-1, DPPH radical scavenging ability was significantly better than that of other groups (P<0.05). It shows a relatively weaker Fe³⁺ reduction ability when incorporate Zn²⁺ into the COS-Zn²⁺. Compared with the normal group, the body weight and viscera index of the model group were significantly changed (P<0.05) in the animal experiment. COS-Zn²⁺administration was helpful to the body weight gain and organ index recovery in mice; COS-Zn²⁺effectively reduced the content of MDA in the model mice and significantly increased the activities of SOD, T-AOC, GSH-Px and CAT in the serum, kidney and liver of the model mice, especially the protective effect of the protective enzymes in serum better than the COS, ZnSO₄ and COS+ZnSO₄ mixture group (P<0.05). COS-Zn²⁺ shows better antioxidant activity in vitro and can significantly enhance the antagonistic ability of mice against oxidative damage. Thus, the COS-Znn2+ showed good anti-aging effect and the enhanced immune function in this study, which can provide a theoretica basis and technical reference for the application and development of maine chitosan oligosaccharides and its metal modifiers.