| 液相色谱-质谱联用测定咖啡中肟菌酯及代谢物残留 |
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| 引用本文： | 张蕊,邓露晴,李祥英,赵俊龙,李梓琳,凡帅,刘承兰.液相色谱-质谱联用测定咖啡中肟菌酯及代谢物残留[J].华南农业大学学报,2019,40(1):72-76. |
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| 作者姓名： | 张蕊 邓露晴 李祥英 赵俊龙 李梓琳 凡帅 刘承兰 |
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| 作者单位： | 天然农药与化学生物学教育部重点实验室/广东省生物农药创制与应用重点实验室/华南农业大学 农学院,广东 广州,510642;天然农药与化学生物学教育部重点实验室/广东省生物农药创制与应用重点实验室/华南农业大学 农学院,广东 广州,510642;天然农药与化学生物学教育部重点实验室/广东省生物农药创制与应用重点实验室/华南农业大学 农学院,广东 广州,510642;天然农药与化学生物学教育部重点实验室/广东省生物农药创制与应用重点实验室/华南农业大学 农学院,广东 广州,510642;天然农药与化学生物学教育部重点实验室/广东省生物农药创制与应用重点实验室/华南农业大学 农学院,广东 广州,510642;天然农药与化学生物学教育部重点实验室/广东省生物农药创制与应用重点实验室/华南农业大学 农学院,广东 广州,510642;天然农药与化学生物学教育部重点实验室/广东省生物农药创制与应用重点实验室/华南农业大学 农学院,广东 广州,510642 |
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| 基金项目： | 农业部农药检定所农药残留项目（31371959） |
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| 摘 要： | 【目的】建立一种同时测定咖啡中肟菌酯及其代谢物肟菌酸残留量的液相色谱–质谱检测方法。【方法】样品中待测农药组分采用含φ为1%乙酸的乙腈匀浆后超声提取,氯化钠和无水硫酸镁盐析及高速离心后,取上清液经C18分散固相萃取净化,采用液相色谱–质谱联用检测,ESI (+)电离和多反应监测(MRM)定量测定。【结果】添加肟菌酯质量分数为0.01～2.00 mg·kg–1时,肟菌酯在咖啡全果中的添加回收率为87.8%～106.7%,相对标准偏差(RSD)为1.3%～5.8%;在咖啡豆中添加回收率为83.2%～88.1%,RSD为2.0%～6.2%。肟菌酸在咖啡全果中的添加回收率为71.5%～106.0%,RSD为1.0%～6.1%;在咖啡豆中的添加回收率为84.4%～105.2%,RSD为1.0%～5.2%。肟菌酯及肟菌酸在咖啡中的最小检出量均为2.5×10–12 g,最低检出限均为0.01 mg·kg–1。【结论】该方法操作简便、快速和稳定,可以满足咖啡实际样品中肟菌酯及其代谢物肟菌酸的残留检测要求。
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| 关 键 词： | 肟菌酯 肟菌酸 液相色谱-质谱联用 农药残留 咖啡 |
| 收稿时间： | 2018/4/23 0:00:00 |
Determination of trifloxystrobin and its metabolite in coffee by liquid chromatography-mass spectrometry |
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| ZHANG Rui,DENG Luqing,LI Xiangying,ZHAO Junlong,LI Zilin,FAN Shuai and LIU Chenglan.Determination of trifloxystrobin and its metabolite in coffee by liquid chromatography-mass spectrometry[J].Journal of South China Agricultural University,2019,40(1):72-76. |
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| Authors: | ZHANG Rui DENG Luqing LI Xiangying ZHAO Junlong LI Zilin FAN Shuai and LIU Chenglan |
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| Institution: | Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education/Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province/College of Agriculture, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education/Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province/College of Agriculture, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education/Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province/College of Agriculture, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education/Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province/College of Agriculture, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education/Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province/College of Agriculture, South China Agricultural University, Guangzhou 510642, China,Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education/Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province/College of Agriculture, South China Agricultural University, Guangzhou 510642, China and Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education/Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province/College of Agriculture, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | |
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| Keywords: | trifloxystrobin trifloxystrobin acid LC-MS pesticide residue coffee |
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