鹅肝酶解物对酒精性脂肪肝的改善作用

为探索鹅肝酶解物对酒精性脂肪肝的防治效果,以6周龄SD大鼠为研究对象,将其随机分为对照组、模型组,以及鹅肝酶解物高(GLH-H)、低剂量处理组(GLH-L),其中对照组以Lieber-DeCarli液体对照饲料喂养,其他各组以Lieber-DeCarli乙醇液体饲料喂养;GLH-H、GLH-L处理组分别给药600、200 mg·kg^-1 BW(体质量)GLH进行干预,对照组和模型组分别给予相同体积的生理盐水灌胃,试验共持续5周。试验结束后测定各试验组大鼠的体质量、肝脏指数、血清中尿素氮、总胆固醇、甘油三酯及炎症因子,分析大鼠肠道微生物的组成,并对各试验组大鼠肝脏病理切片进行观察。结果表明,GLH处理明显降低了酒精性脂肪肝大鼠的肝脏指数(P<0.05),改善了酒精性脂肪肝大鼠肝脏脂肪变性程度,显著降低了大鼠血清中的尿素氮、总胆固醇、甘油三酯及炎症水平(P<0.05)。高通量测序发现,GLH可改善大鼠肠道微生物紊乱的现象。综上,GLH对酒精性脂肪肝的发生具有一定的预防作用。本研究结果为酒精性脂肪肝治疗药物的研究提供了新思路。

Effect of Goose Liver Enzymatic Hydrolysate on Alcoholic Fatty Liver in Rats

This study aimed to explore the preventive effects of goose liver hydrolysate (GLH) on the alcoholic fatty liver (AFL) disease in rats. 6-week-old SD rats were randomly divided into 4 groups with different supplements in Lieber-DeCarli liquid basal diet: control group (normal saline), model group (normal saline + ethanol), GLH-H (600 mg·kg^-1 BW GLH + ethanol) and GLH-L (200 mg·kg^-1 BW GLH + ethanol). The experiment period lasted for 5 weeks. And then for each rat, the body weight, liver index, serum urea nitrogen, total cholesterol, triglyceride and inflammatory factors were measured, the composition of the intestinal microflora was analyzed, and the liver pathological section was prepared and observed. The results showed that GLH treatment improved the degree of hepatic steatosis and significantly reduced the liver index, serum nitrogen, total cholesterol, triglyceride and serum inflammation levels in GLH-H and GLH-L rats, compared to AM rats (P<0.05). At the same time, high-throughput sequencing revealed that GLH could also ameliorate the disorder of intestinal microflora caused by AFL. The results indicate that GLH has preventive effects on the occurrence of AFL disease. This this study would provide new ideas for the exploration of drugs for AFL disease.