马来甜龙竹种胚培养增殖芽芽尖再生体系建立

为建立马来甜龙竹高效稳定的再生体系,本研究以马来甜龙竹种胚培养增殖芽的芽尖为试验材料,研究不同植物激素、有机添加物以及弱光处理对其再生体系建立的影响。结果表明,马来甜龙竹芽尖愈伤组织诱导较适宜培养基为添加0.5~1.0 mg·L^-1 NAA和3 mg·L^-1 2,4-D的MS培养基,致密颗粒状愈伤组织诱导率达61.7%;较适宜的分化培养基为添加1 mg·L^-1 6-BA、1 mg·L^-1 KT和0.25 mg·L^-1 NAA的MS培养基,10 μmol·m^-2·s^-1弱光处理6 d,分化率达46.67%,再生苗伸长生长良好;较适宜的生根培养基为添加3 mg·L^-1 IBA的1/2 MS培养基,生根率为55%,根较粗,其中部分根长有侧根。试管苗移植到混合基质中,成活率为83.33%。本研究初步建立了马来甜龙竹种胚培养增殖芽芽尖再生体系,为马来甜龙竹遗传转化体系的研究和应用奠定了基础。

Establishment of Regeneration System Based on Shoot Tips in Proliferation Buds From Embryo of Dendrocalamus asper

To establish an efficient and stable regeneration system for Dendrocalamus asper, different concentrations and combinations of plant hormone and organic additives, as well as low light treatment were conducted by using shoot tips in proliferation buds from embryo as explants. The results showed that MS medium supplemented with (0.5~1) mg·L^-1 NAA and 3 mg·L^-1 2,4-D was the optimum medium for callus induction, with the frequency of granular and compact callus to 61.7%. The suitable medium for shoot differentiation was MS medium supplemented with 1 mg·L^-1 6-BA, 1 mg·L^-1 KT, and 1 mg·L^-1 NAA, with low light (10 μmol·m^-2·s^-1) treatment for 6 days, with which the highest differentiation rate reached up to 46.67%. The most suitable rooting medium was 1/2 MS medium supplemented with 3 mg·L^-1 IBA with the rooting rate of 55%. The resulting roots were sturdy coupled with lateral roots developed partly. Rooted plantlets were transferred to artificial mixture with the survival rate up to 83.33%. The regeneration system of the shoot tips in proliferation buds from embryo of D. asper was established, providing guidelines for the research and application of the genetic transformation system of Dendrocalamus asper.