| 水稻腥黑粉病菌的单孢检测 |
| |
| 引用本文： | 周业琴,刘素萍,周国梁,易建平,印丽萍,董英. 水稻腥黑粉病菌的单孢检测[J]. 植物检疫, 2006, 0(Z1) |
| |
| 作者姓名： | 周业琴 刘素萍 周国梁 易建平 印丽萍 董英 |
| |
| 作者单位： | 江苏大学 华南农业大学 上海出入境检验检疫局 江苏大学 镇江 |
| |
| 基金项目： | 上海市重大科技攻关项目(03DZ19315和04DZ05002)资助本项目 |
| |
| 摘 要： | 根据水稻腥黑粉菌两个聚类群与其它黑粉菌ITS序列的差异,分别设计了两个聚类群的特异引物Hor2/Hor9和Hm1/Hm5,结合ITS通用引物Til1/Til4建立了分别检测水稻腥黑粉菌两个聚类群单个冬孢子的套式(巢式)PCR检测方法,整个检测过程可以缩短至8h。
|
| 关 键 词： | 水稻腥黑粉菌 套式（巢式）PCR 冬孢子 检测 |
The detection for single teliospore of Tilletia horrida |
| |
| Zhou Yeqin,Liu Suping,Zhou Guoliang,Yi Jianping ,Yin Liping,Dong Ying. The detection for single teliospore of Tilletia horrida[J]. Plant Quarantine, 2006, 0(Z1) |
| |
| Authors: | Zhou Yeqin Liu Suping Zhou Guoliang Yi Jianping Yin Liping Dong Ying |
| |
| Affiliation: | Zhou Yeqin~1,Liu Suping~2,Zhou Guoliang~3,Yi Jianping~ 3,Yin Liping~3,Dong Ying~1 |
| |
| Abstract: | Isolates of Tilletia horrida from different locations were divided into two distinct clades(groups) based on the rDNA ITS sequences,with the sequence difference,the nested PCR methods using group-specific primers Til1/Til4(Hor2/Hor9) and Til1/Til4(Hm1/Hm5) were developed for detecting teliospore of two groups, respectively. One teliospore can be amplified and obtained specific amplicon. The process of detection can be finished within 8 hours. Because of avoidance of DNA extraction,this method can be useful and potential for detection of a few teliospores in imported grain samples. |
| |
| Keywords: | Tilletia horrida nested PCR teliospore detection |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
