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| 罗布麻产黄酮内生真菌的筛选与鉴定 | |
| 引用本文： | 范瑛阁,孙晓东,龚明福. 罗布麻产黄酮内生真菌的筛选与鉴定[J]. 华中农业大学学报, 2013, 32(1): 59-62 |
| 作者姓名： | 范瑛阁 孙晓东 龚明福 |
| 作者单位： | 1. 塔里木大学植物科学学院,阿拉尔843300;新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,阿拉尔843300 2. 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,阿拉尔,843300 3. 乐山师范学院化学与生命学院,乐山,614000 |
| 基金项目： | 国家自然科学基金项目（30960227） |
| 摘 要： | 以罗布麻叶中内生真菌为研究对象,用化学显色法和薄层层析显色法对产生黄酮的内生真菌进行筛选,并根据形态特征对筛选到的内生真菌进行鉴定。结果表明:内生真菌菌株P-1和P-2均能产生黄酮;菌株P-1为镰孢菌(Fusariumsp.),菌株P-2为嗜松青霉(Penicillin pinophilum)。 |
| 关 键 词： | 罗布麻 内生真菌 黄酮 筛选 鉴定 |
| 收稿时间： | 2012-07-16 |
Screening and identification of endophytic fungi produced by flavonoids in Apocynum venetum |
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| FAN Ying-ge , SUN Xiao-dong , GONG Ming-fu. Screening and identification of endophytic fungi produced by flavonoids in Apocynum venetum[J]. Journal of Huazhong Agricultural University, 2013, 32(1): 59-62 | |
| Authors: | FAN Ying-ge SUN Xiao-dong GONG Ming-fu |
| Affiliation: | 1.College of Plant Science and Technology,Tarim University,Alar 843300,China; 2.Key Laboratory of Protection and Utilization of Biological Resources of Xinjiang Production and Construction Corps in Tarim Basin,Tarim University,Alar 843300,China; 3.College of Chemistry and Life,Leshan Normal University,Leshan 614000,China |
| Abstract: | Plant endophytic microorganisms have a close metabolic relationship with its host plants. Endophytic fungi in leaves of Apocynum venetum was used as research material. Endophytic fungi with the capability of producing flavonoids were screened with the methods of chemical colorimetry and thin layer chromatography, and then they were identificated by observing their morphological characteristics. The results showed that two fungal strains named P-1 and P-2 were screened out. They were detected to be able to produce flavonoid compounds. Strain P-1 belongs to Fusarium ,whereas strain P-2 belongs to Penicillin pinophilum . This study laid a foundation for further exploitation of the endophytic fungi in A.venetum . |
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