| 水分胁迫对小麦叶片抗病相关酶活性的诱导 |
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| 引用本文： | 陈 鹏,彭海霞,张 静. 水分胁迫对小麦叶片抗病相关酶活性的诱导[J]. 西北农业学报, 2011, 20(2): 56-51 |
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| 作者姓名： | 陈 鹏 彭海霞 张 静 |
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| 作者单位： | 西北农林科技大学,生命科学学院,陕西杨凌,712100 |
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| 基金项目： | 西北农林科技大学学术骨干支持计划 |
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| 摘 要： | 旨在分析水分胁迫对小麦叶片抗病相关酶的诱导,探讨水分胁迫诱导抗病性的机制。以水分胁迫可诱导其抗病性的小麦品种-小偃6号为材料,用不同质量分数PEG-6000模拟干旱胁迫,采用真空渗透离心法分离胞间隙液以及胞内蛋白,并对不同胁迫时间胞间隙和胞内抗病相关蛋白及酶活性进行分析。结果显示,随着胁迫强度的增加和处理时间的延长,叶片及胞间隙液POD、PPO、PAL(胞间隙除外)、几丁质酶活性均表现出明显的上升的趋势,复水2 d后下降,但各项测定指标均未恢复至对照水平。由此可知,水分胁迫可诱导小偃6号叶片中各种抗病防卫相关酶活性的增强,表明小麦对水分胁迫的响应与抗病信号转导途经之间可能存在共同的机制。
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| 关 键 词： | 水分胁迫 细胞间隙 PAL POD PPO 几丁质酶 |
Drought Induced Enzymes Related with Disease Resistance in Wheat Leaves |
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| CHEN Peng,PENG Haixia and ZHANG Jing. Drought Induced Enzymes Related with Disease Resistance in Wheat Leaves[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2011, 20(2): 56-51 |
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| Authors: | CHEN Peng PENG Haixia ZHANG Jing |
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| Affiliation: | CHEN Peng,PENG Haixia and ZHANG Jing (College of Life Sciences,Northwest A&F University,Yangling Shaanxi 712100,China) |
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| Abstract: | In order to analyse enzymes related with disease resistance in wheat leaves induced by drought,and explore the mechanisms of drought induced disease resistance.Wheat variety Xiaoyan 6 with the character of drought induced stripe rust resistance was treated with different concentrations of PEG-6000 to simulate drought stress.The intercellular washing fluids and intracellular proteins were isolated using vaccum penetration-centrifuge method.Disease-related proteins and enzymes in intercellular washing fluids ... |
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| Keywords: | PAL POD PPO |
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