雷蒙德氏棉PIF-like转座元件鉴定与特征分析

PIF-like转座元件在植物基因组中含量丰富,是植物DNA转座子的重要组成部分。利用基因组学和生物信息学的研究手段,系统研究了雷蒙德氏棉全基因组水平PIF-like转座元件的数量、染色体分布、结构特征、表达模式及相邻基因的种类和功能等,共鉴定了440条包含转座酶且插入位置明确的PIF-like转座元件。这些元件几乎均匀地分布在13条染色体上,上下游2 kb内共有171个相邻编码蛋白的基因,其中132个基因注释到了GO数据库中,且这些基因的功能分布广泛;部分PIF-like转座元件表达,且具有组织特异性。这些遗传信息为深入研究棉花PIF-like转座元件介导的基因和基因组的进化规律以及棉花基因的功能奠定基础。

Genome-wide Identification and Characterization of PIF-like Elements in Gossypium raimondii

PIF-like elements are abundant in plant genomes and make up an important part of DNA transposons. In this study, we systematically studied the number, chromosome distribution, characteristic structure, and expression pattern of PIF-like elements in the Gossypium raimondii genome using genomic and bioinformatic methods. We also studied the types and functions of their adjacent genes. Four hundred and forty PIF-like elements that carried transposase and had clear insert positions were detected. These were distributed almost evenly on 13 chromosomes. One hundred and seventy-one adjacent protein-encoding genes with distances of less than 2 kb to the PIF-like elements were identified, 132 of which were annotated to the Gene Ontology database with a wide variety of functions. Some of the 440 PIF-like elements were expressed, with the expression patterns varying among different tissues. These results will provide a firm foundation for studying the evolution of genes and genomes mediated by PIF-like elements and the functions of protein-encoding genes in cotton.