Review of the Methods for Developing SSR Molecular Markers |
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Authors: | ZHAO Xue CHANG Wei HAN Yingpeng LI Wenbin |
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Institution: | Soybean Research Institute,Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University,Harbin 150030,China |
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Abstract: | Microsatellite marker (or Simple Sequence Repeate,SSR) is a marker technology based on DNA molecular length polymorphism.It is also one of the most commonly used molecular markers.Traditional SSR marker development methods are relatively time-consuming and mostly relying on the known genome sequence information while recently developed methods of SSR marker based on RAPD,ISSR-PCR SSR,the use of hybrid options, sequence tag SSR library access and screening EST-SSR have been widely used.This paper gave an overview of the methods mentioned above for the development of SSR markers. |
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Keywords: | SSR marker development method EST-SSR |
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