Picobirnavirus detection in bovine and buffalo calves from foothills of Himalaya and Central India |
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Authors: | Yashpal Singh Malik K M Chandrashekar Kuldeep Sharma Adil A Haq Nirupama Vaid Somendu Chakravarti Munish Batra Rashmi Singh A B Pandey |
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Institution: | (1) Division of Virology, Indian Veterinary Research Institute (IVRI), Mukteswar, Nainital, Uttarakhand, 263138, India;(2) JN Krishi Vishwa Vidyalaya, Jabalpur, 482001, Madhya Pradesh, India;(3) GB Pant University of Agriculture and Technology, Pantnagar, US Nagar, Uttarakhand, India |
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Abstract: | The present study describes detection of picobirnavirus (PBV) in faecal samples from bovine and buffalo calves employing the
polyacrylamide gel electrophoresis (PAGE). A total of 136 faecal samples from buffalo (n = 122) and cow calves (n = 14) exhibiting clinical signs of diarrhoea and from healthy calves were collected during 2007–2010 from subtropical (central
India) and tarai area of western temperate Himalayan foothills (Uttarakhand). The dsRNA nature of the virus was confirmed
by nuclease treatment (RNase A, RNaseT1 and DNase 1). PAGE results confirmed 3.67% (5/136) positivity for PBV, showing a typical
genomic migration pattern with two discrete bands with size of approximately 2.4 and 1.7 kbps for the larger and smaller segments,
respectively. Among the five PBV samples identified, three were from buffalo calves and one from cow calf exhibiting clinical
signs of acute diarrhoea, while one sample from non-diarrhoeic buffalo calf also showed the presence of PBV. None of the samples
showed dual infection of rotavirus and PBV. The preliminary findings indicate sporadic incidences of PBV in bovine calves
and emphasize the need for the development of better diagnostics for early detection and genetic characterization of these
emerging isolates of farm animals of economic significance. |
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