| 猪IL-18成熟蛋白基因重组真核表达载体的构建及其抗病毒作用研究 |
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| 引用本文： | 曹素芳,王岩.猪IL-18成熟蛋白基因重组真核表达载体的构建及其抗病毒作用研究[J].郑州牧业工程高等专科学校学报,2011,31(3):1-5. |
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| 作者姓名： | 曹素芳 王岩 |
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| 作者单位： | 郑州牧业工程高等专科学校动物医学系,河南郑州,450011 |
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| 基金项目： | 河南省基础与前沿技术研究计划项目(072300430060); 河南省重点科技攻关项目(072102130023); 河南省高校科技创新人才支持计划 |
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| 摘 要： | 应用RT-PCR方法从三元猪脾脏淋巴细胞中扩增出猪白细胞介素-18成熟蛋白(pmIL-18)基因,经克隆测序表明,pmIL-18基因长474bp,编码157个氨基酸。序列分析发现,与已发表的pmIL-18基因序列同源性很高,均为99%以上。将pmIL-18基因插入表达载体pcDNA3.1,成功构建了pmIL-18基因的重组真核表达载体pcD-NA3/pmIL-18。将重组质粒转染COS7细胞,经SDS-PAGE及Western-blotting分析表明,pmIL-18基因在COS7细胞中获得了瞬时表达。收集转染细胞培养上清,检测pmIL-18抗病毒活性,结果表明,转染上清对猪繁殖与呼吸综合征病毒没有明显的抑制作用。
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| 关 键 词： | 猪 白细胞介素-18 真核表达载体 抗病毒活性 |
Construction of eukaryotic expression vector and antiviruses analysis of porcine mature interleukin-18 |
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| Cao Su-fang,Wang Yan.Construction of eukaryotic expression vector and antiviruses analysis of porcine mature interleukin-18[J].Journal of Zhengzhou College of Animal Husbandry Engineering,2011,31(3):1-5. |
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| Authors: | Cao Su-fang Wang Yan |
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| Institution: | Cao Su-fang,Wang Yan(College of Zheng Zhou Animal Husbandry and Engineering,Zhengzhou Henan 450011,China) |
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| Abstract: | After the total RNA extracted from lymphocyte of pig spleen,porcine interleukin-18 mature protein(pmIL-18) gene was amplified by RT-PCR.It was cloned into pGEM-T easy vector and then sequenced.The result showed that the pmIL-18 gene composed 474 bp and the predicted primary protein is composed of 157 amino acide.After comparing with pmIL-18 gene from Genbank,it showed that nucleotide sequence homology was beyond 99%.The f ragment pmIL-18 gene was obtained from the recombinant plasmid pGEM/pmIL-18 by restric... |
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| Keywords: | Porcine Interleukin-18 eukaryotic expression vector antiviruses activity |
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