导入矮秆基因的小偃麦材料的细胞学和储藏蛋白研究

用小麦 -中间偃麦草部分双二倍体与含Rht1 0的小麦品系杂交回交 ,培育了稳定的株高在 5 0～ 70cm左右 ,农艺性状较优的矮杆小偃麦品系。我们对其中的 2份材料进行了细胞学和醇溶蛋白分析。结果表明 ,材料攀890 74- 1 - 1 - 1 - 1、攀 890 74- 3- 2 - 1的染色体数分别为 2n =5 6和 2n =5 0。利用种子醇溶蛋白电泳分析表明它们与“中 5”均具有反应偃麦草供体的特征带 ,在Gli-B1和Gli-D1上表现一定的差异 ,攀 890 74- 3- 2 - 1还含来自黑麦的 1RS蛋白带 ,攀 890 74- 1 - 1 - 1 - 1缺少“中 5”的一条Gli-B1带。在高分子谷蛋白亚基上 ,攀 890 74- 1- 1 - 1和攀 890 71 - 3- 2 - 1与“中 5”在Glu -A1和Glu -B1上表现了较大的差异。结果认为 ,品系攀 890 74- 1 -1 - 1和攀 890 74- 3- 2 - 1是四川及南方麦区小麦育种中利用中间偃麦草基因的优良品种资源

Cytological and Seed Storage Protein Analysis of Dwarf Lines with Wheat-Thinopyrum Intermedium Chromatin

Lines Pan 89074-1-1-1 and Pan 89074-3-2-1, with 5～70cm height,wee developed from a partial amphiploid wheat Thinopyrum intermedium, line with Rht10 gene. Cytological analysis exhibits that the chromosome number of Pan89074-1-1-1-1 and Pan 89074-3-2-1 are 56 and 50 respectively. APAGE of gliadin indicates that both Pan 89074-1-1-1-1and Pan 89074-3-2-1 containe band Gli-Agi1, but Pan 89074-1-1-1-1 is lack of band Gli-B1 than Zhong 5, and Pan 89074-3-2-1 has additional band of Gli-B1l, which shows that Pan 89074-3-2-1 possesses 1RS/1BL chromosome. The differences of HMW-GS between Pan 89074-1-1-1-1, Pan 89074-3-2-1 and Zhong 5 are obtained by SDS-PAGE analysis. The Agronomical traits also showed that lines Pan 89074-1-1-1-1 and Pan 89074-3-2-1 are novel resources for transfer Thinopyrum intermedium chromatin to wheat cultivars in south China.