| 花椰菜种株的组培快繁技术探讨 |
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| 引用本文： | 方淑桂,朱朝辉,林翮飞,陈琰臻.花椰菜种株的组培快繁技术探讨[J].福建农业科技,2013(10):28-30. |
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| 作者姓名： | 方淑桂 朱朝辉 林翮飞 陈琰臻 |
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| 作者单位： | [1]福建省福州市蔬菜科学研究所,350111 [2]福建省福州市农业局,350111 |
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| 基金项目： | 福建省重大专项（2008NZ0002-1）;福建省科技计划重点项目（20080046）;福州市奖励项目. |
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| 摘 要： | 以花椰菜雄性不育系的种株为材料,进行组培快繁技术研究,建立花椰菜种株组培快繁技术体系.试验结果表明：花椰菜的薹茎段是母株组培快繁的理想外植株,其不定芽分化率高达90％;适宜不定芽分化的培养基为MS＋6-BA 2 mg/L＋ NAA 0.1 mg/L;再生植株通过田间生产鉴定与实生母株相比,植株性状、花球性状、育性等都表现完全一致.
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| 关 键 词： | 花椰菜 雄性不育系 种株 组织培养 |
Rapid propagation of cauliflower seed plant by tissue culture |
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| FANG Shu-gui,ZHU Chao-hui,LIN He-fei,CHEN Yan-zhen.Rapid propagation of cauliflower seed plant by tissue culture[J].Fujian Agricultural Science and Technology,2013(10):28-30. |
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| Authors: | FANG Shu-gui ZHU Chao-hui LIN He-fei CHEN Yan-zhen |
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| Institution: | 1. Fuzhou Institute of Vegetable Science, Fujian Province 350111 ; 2. Fuzhou Agricultural Bureau, Fujian Province) |
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| Abstract: | By using seed plant of cauliflower male sterile line as material, the technology system for rapid propagation of cauliflower by tissue culture was studied and established in this research. The results showed that the bolting stem section was the ideal explants, and the differentiation ratio for adventitious buds reached 90%. The optimum medium for adventitious bud differentiation was MS ＋ 6 - BA 2 mg/L ＋ NAA 0.1 mg/L. And the regenerate plant was tested in field to be totally the same as the parent plant in plant characters, head traits, and fertility. |
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| Keywords: | Cauliflower male sterile line seed plant tissue culture |
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