ATP,ADP and AMP dephosphorylation in membrane fractions of <Emphasis Type="Italic">Rhamdia quelen</Emphasis> exposed to different temperatures |
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Authors: | C?L?Lermen V?L?P?Vieira M?R?C?Schetinger R?Lappe C?R?Gioda M?R?Crestani B?Baldisserotto Email author" target="_blank">V?M?MorschEmail author |
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Institution: | (1) Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil;(2) Departamento de Fisiologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil |
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Abstract: | The silver catfish Rhamdia quelen is a teleost species from South America that can resist cold winters and grow quickly in the summer, representing an important
species for aquaculture in both temperate and subtropical climates. NTPDase and 5′-nucleotidase are enzymes responsible for
the sequence of ATP dephosphorylation to adenosine in a variety of organs. This enzymatic cascade permits the resultant adenosine
to be taken up by the cells. In the present study, membrane fractions of brain, liver and kidney from fish Rhamdia quelen exposed to 15, 23 and 31 °C for 21 days (chronic) or 12 h (acute) were incubated with ATP, ADP and AMP as substrates. In
the 21 day experiment the results showed that ATP hydrolysis was enhanced by the increase in temperature in all tissues, except
for 31 °C in the brain. NTPDase hydrolysis using ADP as substrate showed a behavior similar to that observed for ATP hydrolysis,
except for 31 °C, when the behavior was similar to that observed at 15 °C in the brain. 5′-Nucleotidase activity using AMP
as substrate raised with increasing temperature in the three tissues studied. In the 12 h experiment the results obtained
for the three temperatures did not differ significantly from each other for any of the tissues. We conclude that after a period
of 21 days temperature may affect the activity of NTPDase and 5′-nucleotidase, important enzymes implicated in nucleotide
degradation. |
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Keywords: | 5′ -nucleotidase brain kidney liver NTPDase Rhamdia quelen temperature |
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