| 玉米茎腐病拮抗菌筛选及发酵条件优化 |
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| 引用本文： | 王艳红,刘金文,吴志军,李丽阳,于立权,葛文中.玉米茎腐病拮抗菌筛选及发酵条件优化[J].玉米科学,2013,21(2):139-143. |
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| 作者姓名： | 王艳红 刘金文 吴志军 李丽阳 于立权 葛文中 |
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| 作者单位： | 黑龙江八一农垦大学生命科学技术学院, 黑龙江 大庆 163319;吉林省农业科学院, 长春 130033;黑龙江八一农垦大学生命科学技术学院, 黑龙江 大庆 163319;黑龙江八一农垦大学生命科学技术学院, 黑龙江 大庆 163319;黑龙江八一农垦大学生命科学技术学院, 黑龙江 大庆 163319;黑龙江八一农垦大学生命科学技术学院, 黑龙江 大庆 163319 |
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| 基金项目： | 黑龙江省自然科学基金项目(B200812) |
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| 摘 要： | 采用平板稀释法进行菌种选育, 筛选玉米茎腐病的生防菌及对该菌株进行液体发酵。应用响应面法优化发酵条件, 以禾谷镰刀菌为指示菌测定生物活性。筛选得到1株较高活性菌株(YH-1), 将该菌株进行发酵条件优化, 抑菌圈直径由原来的20.60 mm增至25.80 mm。YH-1作为高效玉米茎腐病拮抗菌株, 可为生防菌剂开发奠定良好基础。
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| 关 键 词： | 玉米茎腐病 拮抗作用 Placket-Burman设计 响应面法 |
| 收稿时间： | 9/3/2012 12:00:00 AM |
Screening of Corn Stalk Rot Antagonistic Strain and Optimization of Fermentation |
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| WANG Yan-hong,LIU Jin-wen,WU Zhi-jun.Screening of Corn Stalk Rot Antagonistic Strain and Optimization of Fermentation[J].Journal of Maize Sciences,2013,21(2):139-143. |
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| Authors: | WANG Yan-hong LIU Jin-wen WU Zhi-jun |
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| Institution: | Life Science and Technology College, Heilongjiang Bayi Agricultural University, Daqing 163319;Jilin Academy of Agricultural Sciences, Changchun 130033, China;Life Science and Technology College, Heilongjiang Bayi Agricultural University, Daqing 163319;Life Science and Technology College, Heilongjiang Bayi Agricultural University, Daqing 163319;Life Science and Technology College, Heilongjiang Bayi Agricultural University, Daqing 163319;Life Science and Technology College, Heilongjiang Bayi Agricultural University, Daqing 163319 |
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| Abstract: | In order to screen the corn stalk rot antagonistic strain and study the liquid fermentation, plates dilution method was used for breeding. Fusarium graminearum was used for testing the bioactivities when the response surface method was applied to optimize the fermentation. One strain YH-1 was selected. In comparison to the inhibition zone of original level(20.60 mm), when it was optimized, 25.80 mm inhibition zone was obtained. YH-1 was selected as efficient strain. It established a good foundation for developing biological pesticide. |
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| Keywords: | Corn stalk rot Antagonism Placket-Burman design Response surface method |
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