除虫菊愈伤组织诱导研究

以除虫菊种子获得的无菌苗的不同器官为外植体,研究几种基本培养基及NAA、2,4-D或其与BA、KT的组合对愈伤组织的诱导效果,结果表明：不同类型基本培养基中MS有利于子叶、真叶和下胚轴,White有利于根愈伤组织的诱导和生长。不论除虫菊的哪一部分作为外植体,培养基pH值均为5.8较为合适。不同器官外植体诱导愈伤组织的最适植物生长调节剂组合不同,子叶为1.0 mg/L 2,4-D＋0.5 mg/L KT,真叶为1.0-2.0 mg/L 2,4-D＋0.5 mg/LKT,根为0.5 mg/L 2,4-D＋0.5 mg/L BA或KT,而下胚轴在1.0-2.0mg/L 2,4-D＋0.5 mg/L BA,0.5-1.0 mg/L 2,4-D＋0.5 mg/L KT,1.0 mg/L NAA＋0.5 mg/L BA,0.5mg/L NAA＋0.5 mg/L KT,0.5 mg/L 2,4-D＋0.5 mg/L BA或KT均可100%诱导愈伤组织。

Study on Callus Induction of Pyrethrum cinerariifolium Trev.

The different organs of free seedlings Pyrethrum cinerariifolium seed were used as explants.Effect of different media and pH on callus induction were studied.MS as the basic medium supplemented with NAA,2,4-D,BA,KT,studies were carried out on induction of different explants of P.cinerariifolium.The results showed :MS medium was suitable for the callus induction of cotyledon leaf and hypocotyl of P.cinerariifolium,White was suitable for the callus induction of root.The best pH for the callus induction was 5.8.When cytokinin and auxin were used on callus induction,1.0 mg/L2,4-D 0.5 mg/LKT was best for cotyledon,1.0~2.0 mg/L 2,4-D 0.5 mg/L KT was best for leaves,1.0~2.0 mg/L 2,4-D 0.5 mg/L BA,0.5~1.0 mg/L 2,4-D 0.5 mg/L KT,1.0 mg/L NAA 0.5 mg/L BA,0.5 mg/L NAA 0.5 mg/L KT were best for hypocotyls,0.5 mg/L 2,4-D 0.5 mg/L BA or KT was best for roots,the inductivity could reach at 100 percent whose callus was growing,suitable for subculture.2,4-D was better than NAA,KT was better than BA for callus induction and growth of P.cinerariifolium.