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越橘叶片组培快繁技术研究
引用本文:建德锋. 越橘叶片组培快繁技术研究[J]. 中国果业信息, 2012, 41(5)
作者姓名:建德锋
作者单位:吉林农业科技学院
摘    要:以越橘的叶片为材料进行组培技术研究,结果发现把经过消毒灭菌的叶片切块后接种到1/4Anderson 2,4-D1mg/L TDZ0.1 mg/L培养基上,愈伤组织诱导效果最好,然后把分化出的愈伤组织切割转入1/4Anderson IBA0.1mg/L TDZ1mg/L 2-IP0.5mg/L培养基上进行芽的诱导效果最佳,最好将芽转入1/4Anderson IBA2mg/L或1/4Anderson NAA0.1mg/L培养基上进行生根培养,生根效果较好。

关 键 词:越橘;叶片;培养基;植物激素
收稿时间:2012-05-18
修稿时间:2012-05-18

Leaf Culture of Vaccinium vitis-idaea in Vitro
Abstract:Taking the leaves of Vaccinium vitis-idaea as materials to do the study of culture in vitro, the results showed that the results of callus induction were the best in the medium of 1/4Anderson 2,4-D1mg/L TDZ0.1 mg/L, in which leaf were cut and seeded after disinfection and sterilization. The callus were cut and transferred to the medium of 1/4Anderson IBA0.1mg/L TDZ1mg/L 2-IP0.5mg/L, and the effects of bud induction were the best. At last, the buds were transferred to the medium of 1/4Anderson IBA2mg/L or the medium of 1/4Anderson NAA0.1mg/L to do rooting culture, and the effects were better.
Keywords:Vaccinium vitis-idaea L   leaf   medium   plant hormones
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