越橘叶片组培快繁技术研究 |
| |
引用本文: | 建德锋. 越橘叶片组培快繁技术研究[J]. 中国果业信息, 2012, 41(5) |
| |
作者姓名: | 建德锋 |
| |
作者单位: | 吉林农业科技学院 |
| |
摘 要: | 以越橘的叶片为材料进行组培技术研究,结果发现把经过消毒灭菌的叶片切块后接种到1/4Anderson 2,4-D1mg/L TDZ0.1 mg/L培养基上,愈伤组织诱导效果最好,然后把分化出的愈伤组织切割转入1/4Anderson IBA0.1mg/L TDZ1mg/L 2-IP0.5mg/L培养基上进行芽的诱导效果最佳,最好将芽转入1/4Anderson IBA2mg/L或1/4Anderson NAA0.1mg/L培养基上进行生根培养,生根效果较好。
|
关 键 词: | 越橘;叶片;培养基;植物激素 |
收稿时间: | 2012-05-18 |
修稿时间: | 2012-05-18 |
Leaf Culture of Vaccinium vitis-idaea in Vitro |
| |
Abstract: | Taking the leaves of Vaccinium vitis-idaea as materials to do the study of culture in vitro, the results showed that the results of callus induction were the best in the medium of 1/4Anderson 2,4-D1mg/L TDZ0.1 mg/L, in which leaf were cut and seeded after disinfection and sterilization. The callus were cut and transferred to the medium of 1/4Anderson IBA0.1mg/L TDZ1mg/L 2-IP0.5mg/L, and the effects of bud induction were the best. At last, the buds were transferred to the medium of 1/4Anderson IBA2mg/L or the medium of 1/4Anderson NAA0.1mg/L to do rooting culture, and the effects were better. |
| |
Keywords: | Vaccinium vitis-idaea L leaf medium plant hormones |
|
| 点击此处可从《中国果业信息》浏览原始摘要信息 |
|
点击此处可从《中国果业信息》下载全文 |
|