| 利用PCR技术检测鸡肉产品中的空肠弯曲杆菌 |
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| 引用本文： | 胡哲,王振国,刘金华,蔡阳,肖成蕊,王伟利,徐宝梁. 利用PCR技术检测鸡肉产品中的空肠弯曲杆菌[J]. 吉林农业大学学报, 2005, 27(6): 671-674 |
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| 作者姓名： | 胡哲 王振国 刘金华 蔡阳 肖成蕊 王伟利 徐宝梁 |
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| 作者单位： | 吉林农业大学动物科学技术学院,长春,130118;吉林出入境检验检疫局技术中心,长春,130062;中国检验检疫科学研究院,北京,100025 |
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| 基金项目： | 国家科技部资助项目（2003DIA6N002-1102-03） |
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| 摘 要： | 根据空肠弯曲杆菌旋转酶基因（gyrAgene）的保守序列，设计1对引物，建立了快速检测鸡肉中空肠弯曲杆菌的PCR方法。采用该方法和生化试验对送检的100份鸡肉样品进行检测，结果表明：PCR方法能特异性地扩增出空肠弯曲杆菌192bp核苷酸片段，其它结肠弯曲杆菌、肠炎沙门氏菌、大肠杆菌、副溶血性弧菌、产气荚膜梭菌、绿脓假单胞菌等均不能获得阳性扩增结果。检测限度为8cfu／mL。鸡肉肉水、增菌液和纯培养菌落的PCR鉴定结果，阳性率分别为16％、24％和20％，生化鉴定检出率为20％。
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| 关 键 词： | 空肠弯曲杆菌 PCR 鸡肉 |
| 文章编号： | 1000-5684（2005）06-0671-04 |
| 收稿时间： | 2005-05-17 |
| 修稿时间： | 2005-05-17 |
Detection of Campylobacter jejuni by PCR Method in Chicken Meat Products |
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| HU Zhe, WANG Zhen-guo, LIU Jin-hua, CAI Yang, XIAO Cheng-rui, WANG Wei-li,et al.. Detection of Campylobacter jejuni by PCR Method in Chicken Meat Products[J]. Journal of Jilin Agricultural University, 2005, 27(6): 671-674 |
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| Authors: | HU Zhe WANG Zhen-guo LIU Jin-hua CAI Yang XIAO Cheng-rui WANG Wei-li et al. |
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| Affiliation: | 1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China ; 2. Center of Technology, Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China; 3. Chinese Academy of Inspection and Quarantine, Beijing 100025, China |
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| Abstract: | A rapid polymerase chain reaction(PCR) method was established to detect C.jejuni in chicken meat with a pair of primers designed and synthesized according to the sequence of C.jejuni gyrA gene.100 chicken meat samples were detected with the method and API Campy kit.The result showed that 192 bp was specifically amplified from template of C.jejuni,but did not amplify from those of C.coli,S.enteritidis,E.coli,V.parahaemolyticus,C.perfringens,A.Bacillus,and so on.The limit of the method to detect the bacteria was 8 bacteria cfu/mL.Chicken meat water,enrichment fluid and colony from the pure culture were detected by PCR,and 16%,24%,20% of them were found respectively to be positive for C.jejuni.The detection rate of biochemistry identification is 20%. |
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| Keywords: | Campylobacter jejuni PCR chicken meat |
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