| 引起玉米粗缩病的水稻黑条矮缩病毒山东分离物的分子特性 |
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| 引用本文： | 陈佳,朱芹芹,袁从阳,孙作文,李向东,周涛,范在丰. 引起玉米粗缩病的水稻黑条矮缩病毒山东分离物的分子特性[J]. 植物病理学报, 2008, 38(5): 540-543 |
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| 作者姓名： | 陈佳 朱芹芹 袁从阳 孙作文 李向东 周涛 范在丰 |
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| 作者单位： | 1. 山东农业大学植物保护学院植物病理学系, 泰安 271018;2. 山东省植保总站, 济南 250012;3. 中国农业大学植物病理学系农业部植物病理学重点实验室, 北京 100193 |
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| 基金项目： | 国家公益性行业(农业)科研专项，教育部跨世纪优秀人才培养计划，高等学校博士学科点专项科研项目 |
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| 摘 要： | Four isolates of Rice black-streaked dwarf virus (RBSDV) were collected from the maize plants showing rough dwarf symptom in Linyi and Tai'an,Shandong province.The S10 genomic sequences of these isolates were determined and compared with those of 14 other RBSDV isolates.All of the four sequences were 1 801 base pairs (bp) long including the 5'-UTR of 21 bp and the 3'-UTR of 103 bp.They all contained an open reading frame of 1 677 bp (22-1698),encoding the coat protein (CP) of 558 amino acids.The sequences of these four RBSDV isolates and those of the major cp gene of 14 other isolates available in the GenBank were divided into two groups in the phylogenetic tree.Recombination analysis indicated that the isolate Lym2 was likely a recombinant of isolates Lym1 and Zhjs.
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| 关 键 词： | 水稻黑条矮缩病毒 玉米粗缩病 分子特性 分离物 山东 呼肠孤病毒科 virus 轴对称结构 |
Molecular characterization of Rice black-streaked dwarf virus isolates causing maize rough dwarf disease in Shandong |
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| CHEN Jia,ZHU Qin-qin,YUAN Cong-yang,SUN Zuo-wen,LI Xiang-dong,ZHOU Tao,FAN Zai-feng. Molecular characterization of Rice black-streaked dwarf virus isolates causing maize rough dwarf disease in Shandong[J]. Acta Phytopathologica Sinica, 2008, 38(5): 540-543 |
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| Authors: | CHEN Jia ZHU Qin-qin YUAN Cong-yang SUN Zuo-wen LI Xiang-dong ZHOU Tao FAN Zai-feng |
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| Affiliation: | 1. Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China;2. Plant Protection Station of Shandong Province, Jinan 250012, China;3. Department of Plant Pathology and Key Laboratory of Plant Pathology, Ministry of Agriculture, PRC, China Agricultural University, Beijing 100193, China |
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| Abstract: | Four isolates of Rice black-streaked dwarf virus (RBSDV) were collected from the maize plants showing rough dwarf symptom in Linyi and Tai'an,Shandong province.The S10 genomic sequences of these isolates were determined and compared with those of 14 other RBSDV isolates.All of the four sequences were 1 801 base pairs (bp) long including the 5'-UTR of 21 bp and the 3'-UTR of 103 bp.They all contained an open reading frame of 1 677 bp (22-1698),encoding the coat protein (CP) of 558 amino acids.The sequences of these four RBSDV isolates and those of the major cp gene of 14 other isolates available in the GenBank were divided into two groups in the phylogenetic tree.Recombination analysis indicated that the isolate Lym2 was likely a recombinant of isolates Lym1 and Zhjs. |
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| Keywords: | maize rough dwarf disease Rice black-streaked dwarf virus coat protein phylogenetic analysis recombination |
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