| 烤烟种质资源形态学标记及SSR标记的多样性研究 |
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| 引用本文： | 陈 杰,杨 静,陈建军,赵伟才,蔡一霞,邓世媛,王 维.烤烟种质资源形态学标记及SSR标记的多样性研究[J].华南农业大学学报,2013,34(4):450-457. |
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| 作者姓名： | 陈 杰 杨 静 陈建军 赵伟才 蔡一霞 邓世媛 王 维 |
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| 作者单位： | 1 华南农业大学 农学院，2 贵州省黔东南州烟草公司，;1 华南农业大学 农学院，;1 华南农业大学 农学院，;3 广东省烟草南雄科学研究所，;1 华南农业大学 农学院，;1 华南农业大学 农学院，;1 华南农业大学 农学院， |
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| 基金项目： | 国家烟草专卖局重点科技项目（110200902043）；广东省烟草专卖局(公司)科技项目（200816） |
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| 摘 要： | 田间调查了69份烤烟品种形态学性状，并进行各品种间的遗传距离分析；对69份烤烟种质资源进行SSR分析，估算遗传相似系数；结合这2种方法对69份烤烟资源进行遗传多样性研究.结果表明，69份烤烟种质材料间的遗传距离在0.32~7.83之间，平均为2.80，其遗传相似系数在0.30~0.96之间，平均为0.70.聚类分析结果为，供试品种大致分为国外优质种质及其衍生的后代、国内外地方品种、早花类型品种、晚花类型品种等几个类群.结合形态学标记和分子标记对烤烟种质资源进行遗传多样性评价，揭示了烤烟种间的遗传多样性与亲缘关系.
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| 关 键 词： | 烤烟 种质资源 形态学标记 SSR标记 遗传多样性 聚类分析 |
| 收稿时间： | 2013/1/10 0:00:00 |
The Genetic Diversity of Flue-Cured Tobacco Germplasm Resources Studied by Morphological Markers and SSR Markers |
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| Institution: | 1 College of Agriculture, South China Agricultural University, 2 Qiandongnan Tobacco Company in Guizhou Province,;1 College of Agriculture, South China Agricultural University,;1 College of Agriculture, South China Agricultural University,;3 Nanxiong Guangdong Institute of Tobacco Sciences,;1 College of Agriculture, South China Agricultural University,;1 College of Agriculture, South China Agricultural University,;1 College of Agriculture, South China Agricultural University, |
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| Abstract: | The genetic distance of sixty-nine flue-cured tobacco germplasm resources were analyzed by the field performance characters and SSR markers. The genetic distance was 0.32-7.83 among 69 flue-cured tobacco germplasm varieties, with an average of 2.80. The genetic similarity index was 0.30-0.96 among 69 flue-cured tobacco varieties, with an average of 0.70. Cluster analysis based on the matrix of genetic distance and genetic similarity index, respectively, was performed. Clustering results showed that the tested tobacco varieties could be divided into foreign varieties, domestic varieties, early-flowering type, late-flowering type etc.. The combination of morphological markers and molecular markers to evaluate the genetic diversity of flue-cured tobacco germplasm resources may reveal the interspecific genetic diversity and genetic relationship of tobacco, providing the theory basis for parental selection in cross breeding of tobacco genetic breeding. |
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