Identification and isolation of methionine-cysteine rich proteins in soybean seed |
| |
Authors: | Choon-Joo Kho Benito O de Lumen |
| |
Institution: | (1) Department of Nutritional Sciences, University of California, 94720 Berkeley, CA, USA;(2) Present address: Calgene, Inc., 1920 Fifth Street, 95616 Davis, CA, USA |
| |
Abstract: | We recently developed a method to identify methionine-containing proteins and quantitate their methionine contents 4]. We applied this method to soybeans and identified relatively methionine-rich proteins (MRP) among the albumins. By acidic methanol extraction of the albumins, we obtained a group of low molecular weight methionine-cysteine rich proteins (MCRP) that analyzed 4.0% methionine and 8.8% cysteine. MCRP made up 1–2% of the total protein in soybeans. Reversed-phase HPLC purification of MCRP yielded a protein peak that exhibited a single major band on denaturing polyacrylamide gel electrophoresis, had a molecular weight of 16kD and contained 6.2% methionine and 18.8% cysteine. We are cloning the gene for this protein. Increasing its level through genetic engineering could increase the methionine-cysteine content of soybeans. |
| |
Keywords: | soybean methionine-cysteine rich protein |
本文献已被 SpringerLink 等数据库收录! |
|