核桃体细胞胚发生与转基因研究进展

总结了核桃体细胞胚发生的研究进展，列表统计已报道的核桃５个种和３个杂种体细胞胚发生的外植体与诱导条件，重点论述了影响核桃体细胞胚发生与次生胚发生的因素，介绍了核桃体细胞胚萌发与转化的方法。还总结核桃转基因研究的进展，提出了用核桃体细胞胚发生系统进行外源基因转移的操作模式。

AN OVERVIEW OF PROGRESS ON SOMATIC EMBRYOGENESIS AND TRANSFORMATION IN WALNUT

Significant progress on the somatic embryogenesis and transformation in walnut (Juglans) has been made in the past decade.Somatic embryogenesis has been achieved in 5 species of walnut.Cotyledons and zygotic embryos from immature fruit were routinely used as the explants for inducing somatic embryogenesis of walnut.Repetitive somatic embryogenesis has been used for the clonal production of in vitro plants and for gene transformation and regeneration of transgenic plants.Using Agrobacterium |mediated transformation of somatic embryos as a method,genes of interest have been transformed into walnut.The transgenic plants successfully expressed the transformed genes.An overview of the progress on those areas in walnut was presented in this article.It focuses on the points of induction of somatic embryogenesis and repetitive somatic embryogenesis as well as somatic embryos germination and their conversion.A model of using Agrobacterium |mediated transformation of somatic embryos for the production of transgenic plants in walnut was also presented.